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Primary Antibodies
Caspase-12 Antibody
Background
Three distinct signaling pathways lead to programmed cell death (apoptosis). The death receptor and mitochondrion pathways are the mains, in which the key apoptotic proteases capase-8 and caspase-9, respectively, are involved. The endoplasmic reticulum (ER) stress is the third apoptotic pathway and caspase-12 is involved (1,2). Caspase-12 is localized to the ER but not to cytoplasm or mitochondrion. Caspase-12 is activated by ER stress, including disruption of ER calcium homeostasis, and mediates ER stress-induced apoptosis. Caspase-12 is co-localized to the ER with several proteins that are involved in Alzheimer's disease including g-secretase presenilin and b-amyloid precursor protein (APP). Caspase-12 mediates cytotoxicity induced by amyloid-b. Caspase-12 is ubiquitously expressed in mouse tissues (1).
Additional Names
Caspase-12 (NT), Casp-12
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Description
Left: Western blot analysis of caspase-12 in human (A) and mouse (B) spleen tissue lysates with caspase-12 antibody at 1 μg/ml.
Below: Immunohistochemistry of caspase-12 in human heart tissue with caspase-12 antibody at 10 μg/ml.
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Other Product Images
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Source
Caspase-12 antibody was raised against a peptide corresponding to amino acids 2 to 17 of murine caspase-12.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
Caspase-12 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Mouse Caspase-12 (N-Terminus) Peptide (Cat. No. 2325P)
Application
Caspase-12 antibody can be used for detection of caspase-12 by Western blot at 0.5 to 1 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 2325P - Caspase-12 Peptide
Storage
Caspase-12 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1406 - Mouse Spleen Tissue Lysate
- Cat. No. 1301 - Human Heart Tissue Lysate
- Cat. No. 1306 - Human Spleen Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
2094806
Protein Accession Number
CAA73532
Short Description
(NT) A new caspase
This product belongs to the following categories:
Related Products
References- Nakagawa T, Zhu H, Morishima N, Li E, Xu J, Yankner BA, Yuan J. Caspase-12 mediates endoplasmic-reticulum-specific apoptosis and cytotoxicity by amyloid-β. Nature 2000;403:98-103.
- Mehmet H. Caspases find a new place to hide. Nature 2000;403:29-30
- Van de Craen M, Vandenabeele P, Declercq W, Van den Brande I, Van Loo G, Molemans F, Schotte P, Van Criekinge W, Beyaert R, Fiers W. Characterization of seven murine caspase family members. FEBS Lett 1997;403:61-9 (WD1000)
Datasheet 08-02W
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