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Primary Antibodies
Caspase-1 Antibody
Background
Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens (reviewed in 1 and 2). Members of this subfamily include caspase-1, -4, -5, and -12 and can activate proinflammatory cytokines such as IL-1? and IL-18 (3,4). Caspase-1 was initially identified as an IL-1β-converting enzyme (5); later experiments revealed it to be a mammalian homolog of the C. elegans cell death gene ced-3 (6) whose overexpression can induce apoptosis in fibroblasts (7).
Additional Names
Caspase-1 (CT), ICE, IL-1beta converting enzyme
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Description
Left: Western blot analysis of Caspase-1 in human heart cell lysate with Caspase-1 antibody at (A) 0.5, (B) 1, and (C) 2 μg/ml.
Below: Immunohistochemical staining of human heart tissue using Caspase-1 antibody at 2 μg/ml.
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Other Product Images
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Source
Caspase-1 antibody was raised against a 16 amino acid peptide from near the carboxy-terminus of human Caspase-1.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
Caspase-1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human Casp-1 / ICE / IL-1b converting enzyme (C-Terminus) Peptide (Cat. No. 3459P)
Application
Casp-1 antibody can be used for the detection of Caspase-1 by Western blot at 0.5 μg/ml or Immunohistochemistry. Depending on cell lines or tissues used, non-cleaved or other cleavage products may be observed.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3459P - Casp-1 Peptide
Storage
Caspase-1 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1301 - Human Heart Tissue Lysate
Species Reactivity
H
Protein GI Number
15431328
Protein Accession Number
NP_150634
Short Description
(CT) a caspase in the inflammatory subfamily
This product belongs to the following categories:
Related Products
References- Martinon F and Tschopp J. Inflammatory caspases: linking an intracellular innate immune system to autoinflammatory diseases. Cell 2004; 117:561-74.
- Zhivotovsky B and Orrenius S. Caspase-2 function in response to DNA damage. Biochim. Biophys. Res. Comm. 2005; 331:859-67.
- Kuida K, Lippke JA, Ku G, et al. Altered cytokine export and apoptosis in mice deficient in interleukin-1 β converting enzyme. Science 1995; 267:2000-3.
- Gracie JA, Robertson SE, and McInnes IB. Interleukin-18. J. Leukoc. Biol. 2003; 73:213-224.
Datasheet 08-02W
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