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Primary Antibodies
Calreticulin Monoclonal Antibody
Background
Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin can inhibit the binding of androgen receptor to its hormone-responsive DNA element and can inhibit androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Thus, calreticulin can act as an important modulator of the regulation of gene transcription by nuclear hormone receptors. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin but calreticulin is not a Ro/SS-A antigen. Earlier papers referred to calreticulin as an Ro/SS-A antigen but this was later disproven. Increased autoantibody titer against human calreticulin is found in infants with complete congenital heart block of both the IgG and IgM classes.
Additional Names
RO, CRT, SSA, cC1qR, FLJ26680, CALR
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Description
Western blot analysis using calreticulin monoclonal antibody against Hela cell lysate.
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Clonality / Clone
This is a monoclonal antibody. Clone 1G6A7.
Isotype
IgG2a
Host
Calreticulin monoclonal antibody was raised in mouse.
Please use anti-mouse secondary antibodies.
Immunogen
Synthetic peptide of human Calreticulin.
Application
Western Blot:1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Tested Application
E, WB
Storage
Calreticulin monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1201 - HeLa Cell Lysate
Species Reactivity
H
Formulation
Ascitic fluid containing 0.03% sodium azide.
Purified antibody available at no extra cost.
Protein GI Number
811
Short Description
Calreticulin - A major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum.
This product belongs to the following categories:
Related Products
References- J Biol Chem. 2006 May 5;281(18):12841-8. Epub 2006 Mar 9.
- Biochim Biophys Acta. 2006 May;1760(5):745-53. Epub 2006 Feb 28.
Datasheet 0903-W
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