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Primary Antibodies
CXCL1 Antibody
Background
Chemokines are a group of small (approximately 8 to 14 kD), mostly basic, structurally related molecules that regulate cell trafficking of various types of leukocytes through interactions with a subset of 7-transmembrane, G protein-coupled receptors. Chemokines also play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis. Chemokines are divided into 2 major subfamilies, CXC and CC, based on the arrangement of the first 2 of the 4 conserved cysteine residues; the 2 cysteines are separated by a single amino acid in CXC chemokines and are adjacent in CC chemokines. CXC chemokines are further subdivided into ELR and non-ELR types based on the presence or absence of a glu-leu-arg sequence adjacent and N terminal to the CXC motif.[supplied by OMIM].
Additional Names
CXCL1,chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha),GRO1, GROa, MGSA, MGSA alpha, MGSA-a, NAP-3, SCYB1
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Description
Left: Western blot analysis of CXCL1 expression in intestine lysate using CXCL1 antibody 0.07µg/ml.
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Purification
Rabbit Ig G is purified by peptide affinity chromatography method.
Clonality / Clone
This is a polyclonal antibody.
Host
CXCL1 antibody was raised in rabbit.
Please use anti-rabbit IgG secondary antibodies.
Immunogen
Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human CXCL1.
Application
CXCL1 antibody can be used for Western blot at a suggested dilution at 0.0758µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Tested Application
WB
Buffer
Antibody is supplied in PBS buffer with 2% sucrose. Product may ship lyophilized. To reconstitute add 100μl of distilled water making the final concentration 1 mg/ml.
Storage
Antibody is lyophilized from PBS buffer with 2% sucrose. Add 50 µl of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
Positive Control
- Cat. No. 1308 - Human Small Intestine Tissue Lysate
Species Reactivity
H
Protein Accession Number
NP_001502
Molecular Weight
11kDa
Short Description
Anti-CXCL1
This product belongs to the following categories:
Related Products
References- Omari,K.M., et al., (2006) Glia 53 (1), 24-31
Datasheet 0802-W
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