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Primary Antibodies
CX3CL1 Antibody
Background
Chemokines are a family of proteins associated with the trafficking of leukocytes in immune surveillance and inflammatory cell recruitment. They are classified based on the positions of key cysteine residues. CX3CL1 is a CX3C chemokine known to induce adhesion and migration of leukocytes mediated by a membrane-bound and soluble form respectively. Recent experiments have shown that CX3CL1 can suppress the production of nitrous oxide, interleukin-6, and TNF-α in activated microglia and neuronal cells, suggesting that it may act as an intrinsic inhibitor against neurotoxicity by activated microglia. Its receptor, CX3CR1, also functions as a co-receptor for HIV-1 and HIV-2 envelope fusion and virus infection, which can be inhibited by CX3CL1. CX3CL1 is ofen observed migrating at 80 – 100kDa in SDS-PAGE, presumably due to post-translational modification.
Additional Names
CX3CL1, CX3C, CX3C membrane-anchored chemokine, neurotactin, small
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Description
Left: Western blot analysis of CX3CL1 in C2C12 cell lysate with CX3CL1 antibody at (A) 0.5, (B) 1 and (C) 2 mg/ml.
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Source
CX3CL1 antibody was raised against a peptide corresponding to 18 amino acids near the amino terminus of human CX3CL1 .
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
CX3CL1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
CX3CL1 antibody can be used for detection of CX3CL1 by Western blot at 0.5 – 1 mg/ml dilution. (
Tested Application
E, WB
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 2099P - CX3CL1 Peptide
Storage
CX3CL1 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1285 - C2C12 Cell Lysate
Species Reactivity
H, M
Protein GI Number
1899259
Protein Accession Number
AAB50014
Short Description
CX3CR1 ligand
This product belongs to the following categories:
Related Products
References- Bajetto A, Bonavia R, Barbero S, et al. Chemokines and their receptors in the central nervous system. Front. Neuroendocrinol. 2001; 22:147-84.
- Umehara H, Goda S, Imai T, et al. Fractalkine, a CX3C-chemokine, functions predominantly as an adhesion molecule in monocytic cell line THP-1. Immunol. Cell. Biol. 2001; 79:298-302.
- Mizuno T, Kawanokuchi J, Numata K, et al. Production and neuroprotective functions of fractalkine in the central nervous system. Brain Res. 2003; 979:65-70.
- Combadiere C, Salzwedel K, Smith ED, et al. Identification of CX3CR1. A chemotactic receptor for the human CX3C chemokine fractalkine and a fusion coreceptor for HIV-1. J. Biol. Chem. 1998; 273:23799-804.
Datasheet 08-02W
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