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Primary Antibodies
CTRP3 Antibody
Background
Adipose tissue of an organism plays a major role in regulating physiologic and pathologic processes such as metabolism and immunity by producing and secreting a variety of bioactive molecules termed adipokines (reviewed in 1). One highly conserved family of adipokines is adiponectin/ACRP30 and its structural and functional paralogs, the C1q/tumor necrosis factor-?-related proteins (CTRPs) 1-7 (2). Unlike adiponectin, which is expressed exclusively by differentiated adipocytes, the CTRPs are expressed in a wide variety of tissues (3). An analysis of the crystal structure of adiponectin revealed a structural and evolutionary link between TNF and C1q-containing proteins, suggesting that these proteins arose from a common ancestral innate immunity gene (4). Multiple isoforms of human CTRP3 have been reported. It has been suggested that CTRP3 may play a role in skeletal development (5).
Additional Names
CTRP3 (IN), CORS26
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Description
Left: Western blot analysis of CTRP3 in mouse heart cell lysate with CTRP3 antibody at 1 μg/ml in the (A) absence and (B) presence of blocking peptide.
Below: Immunohistochemistry of CTRP3 in mouse heart tissue with CTRP3 antibody at 2 μg/ml.
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Other Product Images
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Source
CTRP3 antibody was raised against a 16 amino acid peptide from near the center of human CTRP3.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
CTRP3 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human CTRP3 / CORS26 (Intermediate Domain) Peptide (Cat. No. 3565P)
Application
CTRP3 antibody can be used for the detection of CTRP3 by Western blot at 1 - 2 μg/ml.Mouse heart cell lysate can be used as positive control. CTRP3 antibody is human, mouse and rat reactive. These proteins are often highly modified post-translationally and migrate in SDS-PAGE at positions other than their predicted size.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3565P - CTRP3 Peptide
Storage
CTRP3 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1401 - Mouse Heart Tissue Lysate
Species Reactivity
H, M
Protein GI Number
62739183
Protein Accession Number
NP_852100
Short Description
(IN) A novel adipokine
This product belongs to the following categories:
Related Products
References- Fantuzzi G. Adipose tissue, adipokines, and inflammation. J. Allergy Clin. Immunol. 2005; 115:911-9.
- Tsao T-S, Lodish HF, and Fruebis J. ACRP30, a new hormone controlling fat and glucose metabolism. Euro. J. Pharmacol. 2002; 440:213-21.
- Wong GW, Wang J, Hug C, et al. A family of Acrp30/ adiponectin structural and functional paralogs. Proc. Natl. Acad. Sci. USA 2004; 101:10302-7.
- Shapiro L and Scherer PE. The crystal structure of a complement-1q family protein suggests an evolutionary link to tumor necrosis factor. Curr. Biol. 1998; 8:335-8.
Datasheet 08-02W
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