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Primary Antibodies
CTGF Antibody
Background
CTGF Antibody
Source
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hCTGF. Anti-Human CTGF specific antibody was purified by affinity chromatography and then biotinylated.
Conjugate
Biotin
Purification
CTGF antibody was purified by affinity chromatography and then biotinylated.
Clonality / Clone
This is a polyclonal antibody.
Host
CTGF antibody was raised in rabbit.
Please use rabbit secondary antibodies.
Preparation
Lyophilized from sterile-filtered PBS solution.
Application
ELISA:
Direct: To detect hCTGF by direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hCTGF.
Sandwich:
To detect hCTGF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required.
Western Blot:
To detect hCTGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hCTGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Tested Application
E, WB
Storage
CTGF lyophilized antibody is stable for at least 6 months when kept at -20˚C. The biotinylated antibody should be reconstituted to a concentration of 50 μg/ml with sterile PBS solution containing 0.1% BSA. This solution can be stored at 4˚C for at least one month without detectable loss of activity. Frozen aliquots of this solution are stable for at least 6 months when kept at -20˚C. Avoid more than one freeze-thaw cycle.
Species Reactivity
H
Short Description
Biotin - Anti-Human CTGF
This product belongs to the following categories:
Related Products
Datasheet 0903-W
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