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Primary Antibodies
CTGF Antibody
Background
CTGF Antibody
Additional Names
CCN 2, Connective Tissue Growth Factor, Hcs 24, Hypertrophic chondrocyte-specific gene product 24, IGFBP8, Insulin-like Growth Factor-Binding Protein 8, MGC102839, NOV 2
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Description
CTGF (connective tissue growth factor) is a 38kDa, cysteine-rich, secreted peptide. It is a new member of the peptide family that include serum-induced immediate early gene products, a v-src-induced peptide and a putative proto-oncogene. Among the many functions of the CTGF gene family are embryogenesis, wound healing and regulation of extracellular matrix production.
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Source
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hCTGF. Anti-Human CTGF specific antibody was purified by affinity chromatography employing immobilized hCTGF matrix.
Purification
CTGF antibody was purified by affinity chromatography employing immobilized CTGF matrix.
Clonality / Clone
This is a polyclonal antibody.
Host
CTGF antibody was raised in rabbit.
Please use rabbit secondary antibodies.
Preparation
A sterile filtered antibody solution (1 mg/mL) was lyophilized from 0.5X PBS pH 7.2.
Application
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hCTGF (5.0 μg/ml), a concentration of 6.0 - 9.0 μg/ml of this antibody is required.
ELISA:
Indirect:
To detect hCTGF by indirect ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hCTGF.
Sandwich:
To detect hCTGF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required.
Western Blot:
To detect hCTGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hCTGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Tested Application
E, WB, N
Storage
CTGF lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/ml. The antibody is stable for at least six weeks at 2-4˚C when reconstituted.
Species Reactivity
H
Short Description
Anti-Human CTGF
This product belongs to the following categories:
Related Products
Datasheet 1001-W
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