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Primary Antibodies
CRYAB Monoclonal Antibody
Background
Crystallin, alpha B.Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP also known as the HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy.
Additional Names
CRYA2, CTPP2, HSPB5, CRYAB
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Description
Left: Western blot analysis using CRYAB monoclonal antibody against full - length CRYAB - Trx recombinant protein
Below: Immunohistochemical analysis of paraffin - embedded human tissue lung carcinoma showing cytoplasmic location with DAB staining using CRYAB antibody.
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Other Product Images
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Clonality / Clone
This is a monoclonal antibody. Clone 10D5F4.
Isotype
IgG2b/IgG2a
Host
CRYAB monoclonal antibody was raised in mouse.
Please use anti-mouse secondary antibodies.
Immunogen
Purified full length recombinant CRYAB-Trx (aa1-175) is expressed in E.Coli strain BL21 (DE3).
Application
Western Blot:1:500 - 1:2,000
IHC(P):1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Tested Application
E, IHC
Storage
CRYAB monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Species Reactivity
H
Formulation
Ascitic fluid containing 0.03% sodium azide.
Purified antibody available at no extra cost.
Protein GI Number
1410
This product belongs to the following categories:
References- Cell. 2007 Aug 10;130(3):427-39.
- Biochemistry. 2006 Nov 21;45(46):13847-54.
- J Mol Biol. 2007 Sep 14;372(2):470-84.
Datasheet 0903-W
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