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Catalog Number:

46-645


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GeneDireX Molecular Weight Marker


Antibody Production


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Primary Antibodies

CLIC4 Antibody

Background

CLIC4 Antibody.

Additional Names

CLIC4, chloride intracellular channel 4, CLIC4L, DKFZP566G223, FLJ38640, H1, huH1, p64H1 , chloride intracellular channel 4 like

CLIC4 Antibody

Description

Left: Western blot analysis of CLIC4 in human kidney lysate (35μg protein in RIPA buffer) using CLIC4 antibody (0.1μg/mL).

Below: Staining of min6 cells transiently expressing mouse CIC4 (5μg/mL). Nuclear counter staining by Hoechst.

Other Product Images

Source

CLIC4 antibody was raised against a synthetic peptide of CLIC4.

Purification

CLIC4 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality / Clone

This is a polyclonal antibody.

Host

CLIC4 antibody was raised in goat.

Please use anti-goat secondary antibodies.

Application

Peptide ELISA: Antibody detection limit dilution 1:64,000. Approx 30kDa band observed in human kidney and human placenta lysates (calculated MW of 28.8kDa according to NP_039234.1). The observed molecular weight corresponds to earlier findings in litera

Tested Application

E, WB, IF

Buffer

CLIC4 antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Blocking Peptide

Cat. No. 46-645P - CLIC4 Peptide

Storage

Aliquot and store at -20°C. Minimize freezing and thawing.

Positive Control

  1. Cat. No. 1305 - Human Kidney Tissue Lysate

Species Reactivity

H, M, R, D

Protein Accession Number

NP_039234.1

Short Description

CLIC4

This product belongs to the following categories:

Related Products

References

  1. Suh KS, Crutchley JM, Koochek A, Ryscavage A, Bhat K, Tanaka T, Oshima A, Fitzgerald P, Yuspa SH. Reciprocal modifications of CLIC4 in tumor epithelium and stroma mark malignant progression of multiple human cancers. Clin Cancer Res. 2007 Jan 1;13(1):121-31.


Datasheet 09-01W

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