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Catalog Number:

45-399


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GeneDireX Molecular Weight Marker


Antibody Production


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Primary Antibodies

CHMP5 Antibody

Background

CHMP5 Antibody.

Additional Names

CHMP5, chromatin modifying protein 5, HGNC:26942, C9orf83, CGI-34, HSPC177, PNAS-2, SNF7DC2, SNF7 domain containing 2, chromosome 9 open reading frame 83

CHMP5 Antibody

Description

Left: Western blot analysis of CHMP5 in K562 lysate (RIPA buffer, 35μg total protein per lane) using CHMP5 antibody (0.5μg/mL).

Source

CHMP5 antibody was raised against a synthetic peptide of CHMP5.

Purification

CHMP5 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality / Clone

This is a polyclonal antibody.

Host

CHMP5 antibody was raised in goat.

Please use anti-goat secondary antibodies.

Application

Peptide ELISA: Antibody detection limit dilution 1:8,000. Western Blot: Approx 28kDa band observed in lysates of K562. Recommended concentration: 1-3μg/ml.

Tested Application

E, WB

Buffer

CHMP5 antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Blocking Peptide

Cat. No. 45-399P - CHMP5 Peptide

Storage

Aliquot and store at -20°C. Minimize freezing and thawing.

Positive Control

  1. Cat. No. 1204 - K562 Cell Lysate

Species Reactivity

H

Protein Accession Number

NP_057494.2

Short Description

CHMP5

This product belongs to the following categories:

Related Products

References

  1. Ward DM, Vaughn MB, Shiflett SL, White PL, Pollock AL, Hill J, Schnegelberger R, Sundquist WI, Kaplan J. The role of LIP5 and CHMP5 in multivesicular body formation and HIV-1 budding in mammalian cells. J Biol Chem. 2005 Mar 18;280(11):10548-55. Epub 2005 Jan 11.


Datasheet 09-01W

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