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Primary Antibodies
CDKN2A Antibody
Background
CDKN2A Antibody. This antibody is expected to recognize isoform 1 only (NP_000068.1)
Additional Names
CDKN2A, cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4) , HGNC:1787, ARF, CDK4I, CDKN2, CMM2, INK4, INK4a, MLM, MTS1, TP16, p14, p14ARF, p16, p16INK4, p16INK4a, p19 , CDK4 inhibitor p16-INK4, cell cycle negative regulator beta, cyclin-
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Description
Left: Western blot analysis of CDKN2A in HeLa lysate (35μg protein in RIPA buffer) using CDKN2A antibody (1μg/mL).
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Source
CDKN2A antibody was raised against a synthetic peptide near the C-terminus of CDKN2A.
Purification
CDKN2A antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality / Clone
This is a polyclonal antibody.
Host
CDKN2A antibody was raised in goat.
Please use anti-goat secondary antibodies.
Application
Peptide ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approx 16-18kDa band observed in lysates of the cell lines A431 and HeLa (calculated MW of 16.5kDa according to NP_000068.1). Recommended concentration: 0.1-1μg/ml.
Tested Application
E, WB
Buffer
CDKN2A antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Blocking Peptide
Cat. No. 45-033P - CDKN2A Peptide
Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Positive Control
- Cat. No. 1201 - HeLa Cell Lysate
Species Reactivity
H
Protein Accession Number
NP_000068.1
Short Description
(CT) CDKN2A (isoform 1) / p16INK4a
This product belongs to the following categories:
Related Products
References- Mawrin C, Kirches E, Schneider-Stock R, Boltze C, Vorwerk CK, von Mawrin A, Kirches E, Schneider-Stock R, Boltze C, Vorwerk CK, von Mawrin A, Kirches E, Schneider-Stock R, Boltze C, Vorwerk CK, von Mawrin A, Kirches E, Schneider-Stock R, Boltze C, Vorwerk Alterations of cell cycle regulators in gliomatosis cerebri. J Neurooncol. 2005 Apr;72(2):115-22.
Datasheet 08-02W
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