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Primary Antibodies
CD81 Antibody
Background
CD81 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family, a group of cell-surface proteins that are characterized by the presence of four hydrophobic domains and mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. CD81 is a cell surface glycoprotein that associates with integrins and appears to promote muscle cell fusion and support myotube maintenance. Along with SCARB1, LDL-R, and CLDN1, CD81 has been reported to be an entry factor for the Hepatitis C virus. Finally, recent evidence indicates that the CD81 gene is localized in a tumor-suppressor gene region and is thus a candidate gene for malignancies.
Additional Names
CD81, S5.7, TAPA1, TSPAN28
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Description
Left: Western blot analysis of CD81 in human lung tissue lysate with CD81 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking petide.
Below: Immunohistochemistry of CD81 in human lung tissue with CD81 antibody at 5 μg/ml.
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Other Product Images
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Source
CD81 antibody was raised against a 20 amino acid peptide near the amino terminus of human CD81.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
CD81 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
CD81 antibody can be used for detection of CD81 by Western blot at 1 - 2 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 5195P - CD81 Peptide
Storage
CD81 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1302 - Human Lung Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
4757944
Protein Accession Number
NP_004347
Short Description
Tetraspanin 28
This product belongs to the following categories:
References- Oren R, Takahashi S, Doss C, et al. TAPA-1, the target of an antiproliferative antibody, defines a new family of transmembrane proteins. Mol. Cell Biol. 1990; 10:4007-15.
- Charrin S, le Naour F, Silvie O, et al. Lateral organization of membrane proteins: tetraspanins spin their web. Biochem. J. 2009; 420:133-54.
- Mannion BA, Berditchevski F, Kraeft SK, et al. Transmembrane-4 superfamily proteins CD81 (TAPA-1), CD82, CD63, and CD53 specifically associated with integrin alpha 4 beta 1 (CD49d/CD29). J. Immunol. 1996; 157:2039-47.
- Tacvhibana I and Hemler ME. Role of transmembrane 4 superfamily (TM4SF) proteins CD9 and CD81 in muscle cell fusion and myotube maintenance. J. Cell Biol. 1999; 146:893-90
Datasheet 09-01W
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