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Primary Antibodies
CD4 Monoclonal Antibody
Background
The CD4 antigen is highly expressed on human T helper cells and thymocytes, and at lower levels on monocytes and dendritic cells. This integral membrane glycoprotein of approximately 58 kDa contains four external domains (D1 to D4) that show homology to members of the immunoglobulin (Ig) superfamily. It is responsible for the recognition of the MHC class II antigen. The CD4 antibody recognizes most thymocytes and about 65% of peripheral blood T cells.
Additional Names
CD4mut
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Description
Flow cytometric analysis of peripheral blood T cells using monoclonal antibody to human CD4.
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Purification
Antibody is purified by protein A affinity chromatography.
Clonality / Clone
This is a monoclonal antibody. Clone B486A1.
Isotype
IgG1
Host
CD4 monoclonal antibody was raised in mouse.
Please use anti-mouse secondary antibodies.
Immunogen
Ni-NTA purified recombinant human CD4 expressed in E. Coli strain BL21 (DE3).
Application
Western Blot, IHC and ELISA.
Dilution:
Western Blot:1:500 - 1,000
ELISA:Determining optimal working dilutions by titration test.
Tested Application
E, WB, IHC
Storage
CD4 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1371 - Human peripheral blood leukocyte (PBL) Lysate
Species Reactivity
H
Formulation
Purified antibody in PBS containing 0.03% sodium azide
Protein GI Number
920
Short Description
Highly expressed on human T helper cells and thymocytes
This product belongs to the following categories:
Related Products
References- M Benkirane J Virol. 1995 November; 69(11): 6898¨C6903.
Datasheet 0903-W
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