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Primary Antibodies
CD31 Monoclonal Antibody
Background
CD31, also known as platelet endothelial cell adhesion molecule 1 (PECAM1), is a type I integral membrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors.It is constitutively expressed on the surface of endothelial cells, and concentrated at the junction between them. The antibody reacts with the murine form of the Platelet-Endothelial Cell Adhesion Molecule. The reactivity of the antibody is restricted to the isoform of the molecule that is selectively expressed by endothelial cells.The antigen is predominantly present at the lateral borders of endothelial cells as described for human PECAM-1.It is also weakly expressed on many peripheral lymphoid cells and platelets.CD31 has been used to measure angiogenesis in association with tumor recurrence. Other studies have also indicated that CD31 and CD34 can be used as markers for myeloid progenitor cells and recognize different subsets of myeloid leukemia infiltrates (granular sarcomas).
Additional Names
CD31, PECAM-1, PECAM1
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Description
Left: Western blot analysis using CD31 monoclonal antibody against truncated CD31 recombinant protein.
Below: Immunohistochemical analysis of paraffin - embedded human lymphonodus tissue showing cytoplasmic location of vascular endothelial cells using CD31 antibody with DAB staining.
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Other Product Images
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Clonality / Clone
This is a monoclonal antibody. Clone 2F7B2.
Isotype
IgG1
Host
CD31 monoclonal antibody was raised in mouse.
Please use anti-mouse secondary antibodies.
Immunogen
Ni-NTA purified recombinant CD31 expressed in E. Coli strain BL21 (DE3).
Application
Western Blot:1:500 - 1:2,000
IHC(P):1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Tested Application
E, WB, IHC
Storage
CD31 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Species Reactivity
H
Formulation
Ascitic fluid containing 0.03% sodium azide.
Purified antibody available at no extra cost.
Protein GI Number
5175
Short Description
Platelet endothelial cell adhesion molecule 1
This product belongs to the following categories:
References- Mayr U et al. Circ Res 98:412-20 (2006).
- Bingle L et al. Br J Cancer 94:101-7 (2006).
- Wynne F et al. Reproduction 131:721-32 (2006).
Datasheet 0903-W
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