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Primary Antibodies
CD14 Monoclonal Antibody
Background
CD14 antigen is a GPI-linked glycoprotein with a molecular weight of 55kD. The CD14 antigen is expressed on cells of the myelomonocytic lineage including monocytes, macrophages and Langerhans cells. Low expression is observed on neutrophils and on human B cells. CD14 antigen is a receptor for bacterial lipopolysaccharide (LPS, endotoxin) and the lipopolysaccharide binding protein (LBP). LBP and CD14 antigen serves two physiological roles. These proteins act as opsonin and opsonic receptor, respectively, to promote the phagocytic uptake of bacteria or LPScoated particles by macrophages.
Additional Names
CD14
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Description
Left: Immunohistochemical analysis of paraffin - embedded human normal lymphnode showing membrane localization using CD14 antibody with DAB staining
Below: Flow cytometic analysis of Human PBMC using monoclonal antibody to human CD14.
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Other Product Images
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Purification
Antibody is purified by protein G affinity chromatography and resuspended in PBS with 0.2%BSA,
0.09% sodium azide. No reconstitution is necessar Concentration is 0.4mg/ml.
Clonality / Clone
This is a monoclonal antibody. Clone 5A3H5D5 (FC), 5A3B11B5,.
Isotype
IgG1
Host
CD14 monoclonal antibody was raised in mouse.
Please use anti-mouse secondary antibodies.
Immunogen
Ni-NTA purified recombinant human CD14 expressed in E. Coli strain BL21 (DE3).
Application
IHC:Dilution 1:200 - 1:400
ELISA:Determining optimal working dilutions by titration test.
Tested Application
IHC, E
Storage
CD14 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Species Reactivity
H
Formulation
Purified antibody in PBS containing 0.03% sodium azide
Protein GI Number
929
Short Description
CD14 antigen is a GPI-linked glycoprotein
This product belongs to the following categories:
Related Products
References- J Mammary Gland Biol Neoplasia 2000, 5 : 227-241.
- J Mammary Gland Biol Neoplasia 2000, 5 : 165-185.
Datasheet 0903-W
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