|
Primary Antibodies
CARD9 Antibody
Background
Apoptosis is related to many diseases and development. Cell death signals are transduced by death domain (DD), death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. CARD containing proteins include some caspases, Apaf-1, CARD4, IAPs, RICK, ARC, RAIDD, BCL-10, and ASC. A novel CARD-containing protein was recently identified and designated CARD9, which interacts with the CARD activation domain of BCL-10 (1). CARD9 associates with BCL-10 and forms a complex within cells. CARD9 induces apoptosis and activates NF-κB. CARD9 is an upstream activator of BCL-10 and NF-κB signaling.
Additional Names
CARD9
|
Description
Left: Western blot analysis of CARD9 expression in human MDA-MB-361 (A) and PC-3 (B) cell lysate with CARD9 antibody at 2.5 μg /ml.
Below: Immunocytochemistry of CARD9 in K562 cells with CARD9 antibody at 10 μg/ml.
|
|
Other Product Images
|
Source
CARD9 antibody was raised against a synthetic peptide corresponding to amino acids 521 to 536 of human CARD9 The sequence is different from that of rat origin by two amino acids.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
CARD9 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human CARD9 Peptide (Cat. No. 2515P)
Application
CARD9 antibody can be used for detection of CARD9 by Western blot at 0.5 to 1 μg/ml. A band at approximately 59 kDa can be detected.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 2515P - CARD9 Peptide
Storage
CARD9 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1216 - PC-3 Cell Lysate
- Cat. No. 1217 - MDA-MB-361 Cell Lysate
- Cat. No. 1204 - K562 Cell Lysate
Species Reactivity
H
Protein GI Number
11066983
Protein Accession Number
AF311287
Short Description
A new CARD-containing protein
This product belongs to the following categories:
Related Products
References- Bertin J, Guo Y, Wang L, Srinivasula SM, Jacobson MD, Poyet JL, Merriam S, Du MQ, Dyer MJ, Robison KE, DiStefano PS, Alnemri ES. CARD9 is a novel caspase recruitment domain-containing protein that interacts with BCL10/CLAP and activates NF-κ B. J Biol Chem. 2000;275(52):41082-6. (RD0202)
Datasheet 08-02W
|
|
