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Primary Antibodies
CARD8 Antibody
Background
Apoptosis is related to many diseases and development. Cell death signals are transduced by death domain (DD), death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. CARD containing proteins include some caspases, Apaf-1, CARD4, IAPs, RICK, ARC, RAIDD, BCL-10, and ASC. A novel CARD-containing protein was recently identified and designated CARD8. This protein interacts with DRAL, a p53-responsive protein implicated in the induction of apoptosis (1,2), and caspase-1 and its related proteins ICEBERG and pesudo-ICE (3). Although there are conflicting reports on whether CARD8 acts a pro- or anti-apoptotic protein (2,4), it has been suggested that it functions as an adaptor molecule regulating caspase-1 and NF-κB activation.
Additional Names
CARD8, Cardinal/TUCAN
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Description
Left: Western blot analysis of CARD8 expression in K562 cell lysate with CARD8 antibody at (A) 2 and (B) 4 μg /ml.
Below: Immunocytochemistry of CARD8 in K562 cells with CARD8 antibody at 10 μg/ml.
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Other Product Images
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Source
CARD8 antibody was raised against a synthetic peptide corresponding to amino acids at the C-terminus of human CARD8.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
CARD8 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human CARD8 / Cardinal / TUCAN Peptide (Cat. No. 3187P)
Application
CARD8 antibody can be used for detection of CARD8 by Western blot at 2 μg/ml. An approximate 45 kDa can be detected.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3187P - CARD8 Peptide
Storage
CARD8 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1204 - K562 Cell Lysate
Species Reactivity
H
Protein GI Number
12247742
Protein Accession Number
AAG50014
Short Description
A new CARD containing protein
This product belongs to the following categories:
Related Products
References- Scholl FA, McLoghlin P, Ehler E, et al. DRAL is a p53-responsive gene whose four and a half LIM domain protein product induces apoptosis. J. Cell Biol. 2000; 151:495-506.
- Stil R, Leonardi A, Formisano L, et al. TUCAN/CARDINAL and DRAL participate in a B activation. FEBS 2002;kcommon pathway for modulation of NF- 521:165-9.
- Razmara M, Srinivasula SM, Wang L, et al. CARD-8 protein, a new CARD family member that regulates caspase-1 activation and apoptosis. J. Biol. Chem. 2002; 277:13952-8.
- Pathan N, Marusawa H, Krajewska M, et al. TUCAN, an antiapoptotic caspase-assoicated recruitment domain family protein overexpressed in cancer. J. Biol. Chem. 2001; 276:32220-9.
Datasheet 08-02W
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