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Primary Antibodies
CARD10 Antibody
Background
Apoptosis is related to many diseases and development. Cell death signals are transduced by death domain (DD), death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. CARD containing proteins include some caspases, Apaf-1, CARD4, IAPs, RICK, ARC, RAIDD, Bcl-10, and ASC. A novel CARD-containing protein was recently identified and designated CARD10 (1). This protein belongs to the membrane-associated guanylate kinase-like (MAGUK) family of proteins that can function as molecular scaffolds that assist assembly of signal transduction molecules (2). CARD 10 interacts with Bcl-10, a Bcl protein that promotes apoptosis, caspase-9 maturation and activation of NF-kappaB (1,3). CARD 10 and the related protein CARMA1 also associate with NEMO, the regulatory subunit of the IkappaK complex (4), demonstrating its importance in the regulation of NF-kappaB transcription factor activation.
Additional Names
CARD10, Caspase recuitment domain family 10 / CARMA3 / Bimp1
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Description
Left: Western blot analysis of CARD10 expression in EL4 cell lysate with CARD10 antibody at (A) 5 and (B) 10 μg /ml.
Below: Immunocytochemistry of CARD10 in EL4 cells with CARD10 antibody at 10 μg/ml.
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Other Product Images
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Source
CARD10 antibody was raised against a synthetic peptide corresponding to 14 amino acids at the C-terminus of human CARD10.
Purification
immunoaffinity purified
Clonality / Clone
This is a polyclonal antibody.
Host
CARD10 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Murine CARD10 / Caspase recuitment domain family 10 / CARMA3 / Bimp1 Peptide (Cat. No. 3013P)
Application
CARD10 antibody can be used for detection of CARD10 by Western blot at 5 - 10 μg/ml.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3013P - CARD10 Peptide
Storage
CARD10 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1287 - EL4 Cell Lysate
Species Reactivity
H, M
Protein GI Number
13488607
Protein Accession Number
AAK26165
Short Description
Caspase recuitment domain family 10
This product belongs to the following categories:
Related Products
References- Wang L, Guo Y, Huang W-J, et al. CARD10 is a novel caspase recruitment domain/membrane-associated guanylate kinase family member that interacts with BCL10 and activates NF-?B. J. Biol. Chem. 2001; 276:21405-9.
- Fanning AS and Anderson JM. Protein modules as organizers of membrane structure. Curr. Opin. Cell Biol. 1999; 11:432-9.
- Fischer KD, Tedford K, Wirth T. New roles for Bcl10 in B-cell development and LPS response. Trends Immunol. 2004; 25:113-6.
- Stilo R, Liguoro D, Di Jeso B, et al. Physical and functional interaction of CARMA1 and CARMA3 with I? kinase ?-NF-?B essential modulator. J. Biol. Chem. 2004; 279:34323-31.
Datasheet 08-02W
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