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Primary Antibodies
CAPN6 Antibody
Background
Calpains make up a ubiquitously expressed, well-conserved family of calcium-dependent cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. This large subunit possesses a cysteine protease domain, and both subunits possess calcium-binding domains. Calpains have been implicated in neurodegenerative processes as their activation can be triggered by calcium influx and oxidative stress. Calpain 6 (CAPN6) is most similar to Calpain 5; the C-terminal region of CAPN6 lacks homology to the calmodulin-like domain of other vertebrate calpains. CAPN6 is thought to be involved in the regulation of microtubule dynamics and cytoskeletal organization. CAPN6 has also been recently identified as an HIV dependency factor (HDF), suggesting that CAPN6 may be an important drug target in HIV treatment.
Additional Names
CAPN6, Calpain 6, CANPX, CalpM
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Description
Left: Western blot analysis of CAPN6 in rat lung tissue lysate with CAPN6 antibody at (A) 0.5 and (B) 1 μg/ml.
Below: Immunohistochemistry of CAPN6 in human lung tissue with CAPN6 antibody at 2.5 μg/ml.
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Other Product Images
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Source
CAPN6 antibody was raised against a 18 amino acid peptide from near the carboxy terminus of human CAPN6.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
CAPN6 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
CAPN6 antibody can be used for the detection of StrepII by Western blot at 0.5 – 1 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 4759P - CAPN6 Peptide
Storage
CAPN6 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1462 - Rat Lung Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
13186316
Protein Accession Number
NP_055104
Short Description
Calpain 6
This product belongs to the following categories:
Related Products
References- Croall DE and Ersfeld K. The calpains: modular designs and functional diversity. Genome Biol. 2007; 8:216.
- Dear N, Matena K, Vingron M, et al. A new subfamily of vertebrate calpains lacking a calmodulin-like domain: implications for calpain regulation and evolution. Genomics 1997; 45:175-84.
- Tonami K, Kurihara Y, Aburatani J, et al. Calpain 6 is involved in microtubule stabilization and cytoskeletal organization. Mol. Cell. Biol. 2007; 27:2548-61.
- Brass AL, Dykxhoorn DM, Benita Y, et al. Identification of host proteins required for HIV infection through a functional genomic screen. Science 2008; 319:921-6.
Datasheet 08-04W
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