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Recombinant Proteins
CALR Recombinant Protein
Background
Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium.
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Description
SDS PAGE: Analysis of CALR Recombinant Protein. 4-20% SDS gradient gel. Coomassie blue staining.
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Source
E. coli
Tested Application
E, WB, MS
Buffer
10 mM Tris, pH 8.0, 0.002% NaN3, 3mM NaCl, 2.5mM
Storage
Store at -70˚C. As with any protein, exposing CALR recombinant protein to repeated freeze/thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
Species Reactivity
n/aH
Protein GI Number
4757900
Protein Accession Number
NP_004334
Domain Information
aa. 18-417
Molecular Weight
46 kDa (Calculated)
Fusion Partner
His-tag at N-terminus
Purity
~90%
Short Description
calreticulin, Sicca syndrome antigen A, autoantigen Ro,
This product belongs to the following categories:
Related Products
References- Horibe,T., Matsui,H., Tanaka,M., Nagai,H., Yamaguchi,Y., Kato,K. and Kikuchi,M. Gentamicin binds to the lectin site of calreticulin and inhibits its chaperone activity Biochem. Biophys. Res. Commun. 323 (1), 281-287 (2004)
Datasheet 0803-W
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