|
Primary Antibodies
Bub1 Antibody
Background
Bub1 was initially identified as the mammalian homolog to the yeast protein through a cDNA screen. Bub1 encodes a kinase involved in the formation of the spindle checkpoint, an important mechanism that ensures high fidelity mitotic chromosome segregation. It is thought to be required for assembly of a functional inner centromere, sister chromatid cohesion via targeting of the Shugoshim protein and metaphase congression. Bub1 functions by phosphorylating cdc20, a member of the mitotic checkpoint complex and activating the spindle checkpoint. A related protein kinase Bub3 interacts with Bub1 and targets it to kinetochores prior to chromosome alignment. Mutations in bub1 have been associated with aneuploidy and several forms of cancer.
Additional Names
Bub1 (NT), Budding uninhibited by benzimidazoles 1
|
Description
Left: Western blot analysis of bub1 in A-20 lysate with bub1 antibody at (A) 2 and (B) 4 μg/ml.
Below: Immunocytochemistry of Bub1 in A20 cells with Bub1 antibody at 10 μg/ml.
|
|
Other Product Images
|
Source
Bub1 antibody was raised against a 19 amino acid peptide from near the amino terminus of human Bub1.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
Bub1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
Bub1 antibody can be used for detection of bub1 by Western blot at 2 – 4 μg/ml.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 4229P - Bub1 Peptide
Storage
Bub1 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1288 - A-20 Cell Lysate
Species Reactivity
H, M
Protein GI Number
8134347
Protein Accession Number
O43683
Short Description
(NT) a kinase involved in spindle formation
This product belongs to the following categories:
Related Products
References- Pangilinan F, Li Q, Weaver T, et al. Mammalian BUB1 protein kinases: map positions and in vivo expression. Genomics 1997; 46:379-88.
- Williams GL, Roberts TM and Gjoerup OV. Bub1: Escapades in a cellular world. Cell Cycle 2007; 6:1699-704.
- Meraldi P and Sorger PK. A dual role for Bub1 in the spindle checkpoint and chromosome congression. EMBO J. 2005; 24:1621-33.
- Tang Z, Shu H, Oncel D, et al. Phosphorylation of cdc20 by bub1 provides a catalytic mechanism for APC/C inhibition by the spindle checkpoint. Mol. Cell 2004; 16:387-97.
Datasheet 08-02W
|
|
