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Primary Antibodies
Bst2 Antibody
Background
Bst2 was first identified as a novel terminal B-cell-restricted antigen termed HM1.24 that is expressed on certain bone marrow cells as well as other tissues. The surface expression of Bst2 on fibroblast cell lines facilitated the stromal cell-dependent growth of DW34, a pre-B-cell line, suggesting that this protein is involved in pre-B-cell growth. Later experiments also showed it to be upregulated in several myeloma cells. More recently, Bst2 was identified an inhibitor of retroviral release from human cells whose activity is antagonized by the HIV-1 accessory protein Vpu. While Bst2 caused retention of virions on cell surfaces or endocytosis into Bst2-positive compartments, its depletion abolished the viral requirement for Vpu for virus release. This activity may represent a potential therapeutic strategy for the treatment of HIV/AIDS.
Additional Names
Bst2 (NT), Bone marrow stromal cell antigen 2, CD317, HM1.24, tetherin
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Description
Left: Western blot analysis of Bst2 in Daudi cell lysate with Bst2 antibody at (A) 1 and (B) 2 μg/ml.
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Source
Bst2 antibody was raised against an 18 amino acid peptide near the amino terminus of the mouse Bst2.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
Bst2 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
Bst2 antibody can be used for detection of Bst2 by Western blot at 1 – 2 μg/ml.
Tested Application
E, WB
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 4661P - Bst2 Peptide
Storage
Bst2 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1224 - Daudi Cell Lysate
Species Reactivity
H, M
Protein GI Number
56971124
Protein Accession Number
AAH87949
Short Description
(NT) Bone marrow stromal cell antigen 2
This product belongs to the following categories:
Related Products
References- Goto T, Kennel SJ, Abe M, et al. A novel membrane antigen selectively expressed on terminally differentiated human B cells. Blood 1994; 84:1922-30.
- Ishikawa J, Kaisho T, Tomizawa H, et al. Molecular cloning and chromosomal mapping of a bone marrow stromal cell surface gene, BST2, that may be involved in pre-B-cell growth. Genomics 1995; 26:527-34.
- Ohtomo T, Sugamata Y, Ozaki Y, et al. Molecular cloning and characterization of a surface antigen preferentially overexpressed on multiple myeloma cells. Biochem. Biophys. Res. Commun. 1999; 258:583-91.
- Neil SJ, Zang T, and Bieniasz PD. Tetherin inhibits retrovirus release and is antagonized by HIV-1 Vpu. Nature 2008; 451:425-30.
Datasheet 08-03W
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