|
Primary Antibodies
Bora Antibody
Background
Bora (Protein aurora borealis) is a key activator of Aurora Related Protein Kinase A (ARK-1), which is a centrosome-associated serine/threonine kinase that regulates centrosome maturation, bipolar spindle assembly and chromosome segregation during mitosis. Bora is localized to the nucleus until mitosis is initiated, then translocates to the cytoplasm in a Cdc2 dependent manner. Activation of Cdc2 initiates the release of Bora into the cytoplasm where it can bind and activate ARK-1. PLK1 (polo-like kinase-1) interacts with Bora to control the accessibility of its activation loop for phosphorylation and activation by ARK-1. Bora and ARK-1 cooperatively activate PLK1 and control mitotic entry. Bora mutants result in multipolar spindles in mitosis identical to those observed when ARK-1 function is blocked. Thus, the ARK1-Bora-PLK1 regulatory circuit in mammalian cells elucidates a key mechanism in cell cycle regulation. At least three isoforms of Bora are known to exist.
Additional Names
Bora (NT), Protein aurora borealis, C13orf34, Chromosome 13 open reading frame 34
|
Description
Left: Western blot analysis of Bora in Jurkat cell lysate with Bora antibody at (A) 1 and (B) 2 μg/ml.
Below: Immunohistochemistry of Bora in mouse brain tissue with Bora antibody at 2.5 μg/ml.
|
|
Other Product Images
|
Source
Bora antibody was raised against a 15 amino acid peptide from near the amino terminus of human Bora.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
Bora antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
Bora antibody can be used for detection of Bora by Western blot at 1-2 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 5117P - Bora Peptide
Storage
Bora antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1205 - Jurkat Cell Lysate
Species Reactivity
H, M, R
Protein GI Number
74737659
Protein Accession Number
Q6PGQ7
Short Description
(NT) Protein aurora borealis
This product belongs to the following categories:
Related Products
References- Berdnik D and Knoblich JA. Drosophila Aurora A is required for centrosome maturation and Actin-dependent asymmetric protein localization during mitosis. Curr. Biol. 2002; 12:640-647.
- Wiese C and O’Brien LL. What's so Bor(a)ing about Aurora A activation? Dev. Cell 2006; 11:133-134.
- Hutterer A, Berdnik D, Wirtz-Peitz F, et al. Mitotic activation of the kinase Aurora A requires its binding partner Bora. Dev. Cell 2006; 11:147-157.
- Fu J, Bian M, Jiang Q, et al.. Roles of Aurora kinases in mitosis and tumorigenesis. Mol. Cancer Res. 2007; 5:1-10.
Datasheet 09-01W
|
|
