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Primary Antibodies
Bonzo Antibody
Background
Human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV) require coreceptors, in addition to CD4, to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, and CCR2b in the chemokine receptor family have been identified as HIV coreceptors. An orphan G protein-coupled receptor was recently cloned and designated Bonzo, STRL33 and TYMSTR, and identified as HIV and SIV coreceptor (1-4). Bonzo/STRL33 serves as coreceptor for SIV, HIV-2 and HIV-1. The messenger RNA of Bonzo/STRL33 is expressed in lymphoid tissues and activated peripheral blood lymphocytes.
Additional Names
Bonzo (NT2), STRL33
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Description
Left: Western blot analysis of Bonzo in human spleen tissue lysate in the absence (lane A) or presence (lane B) of peptide with Bonzo antibody (NT2) at 1:500 dilution.
Below: Immunohistochemistry of Bonzo in human spleen cells with Bonzo antibody at 20 μg/ml.
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Other Product Images
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Source
Bonzo antibody was raised against a peptide corresponding to amino acids near the amino terminus of human Bonzo/STRL33. The sequence of this peptide differs from those of African green monkey and pig-tailed macaque by one or two amino acids, respectively,.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
Bonzo antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human Bonzo (N-Terminus 2) Peptide (Cat. No. 2209P)
Application
Bonzo antibody can be used for detection of Bonzo by Western blot at 1:500 dilution.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 2209P - Bonzo Peptide
Storage
Bonzo antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1306 - Human Spleen Tissue Lysate
Species Reactivity
H
Protein GI Number
2209284
Protein Accession Number
U73529
Short Description
(NT2) HIV coreceptor
This product belongs to the following categories:
Related Products
References- Deng HK, Unutmaz D, KewalRamani VN, Littman DR. Expression cloning of new receptors used by simian and human immunodeficiency viruses. Nature 1997;388:296-300
- Liao F, Alkhatib G, Peden KW, Sharma G, Berger EA, Farber JM. STRL33, A novel chemokine receptor-like protein, functions as a fusion cofactor for both macrophage-tropic and T cell line-tropic HIV-1. J Exp Med 1997;185:2015-23
- Alkhatib G, Liao F, Berger EA, Farber JM, Peden KW. A new SIV co-receptor, STRL33. Nature 1997;388:238
- Loetscher M, Amara A, Oberlin E, Brass N, Legler D, Loetscher P, D'Apuzzo M, Meese E, Rousset D, Virelizier JL, Baggiolini M, Arenzana-Seisdedos F, Moser B. TYMSTR, a putative chemokine receptor selectively expressed in activated T cells, exhibits HIV-1 coreceptor function. Curr Biol 1997;7:652-60 (RD0400)
Datasheet 08-02W
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