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Primary Antibodies
Blimp-1 Antibody
Background
Blimp-1 was initially identified as a zinc finger-containing protein that drives the maturation of B lymphocytes into immunoglobulin-secreting cells. Together with X-box-binding protein 1 (XBP1), Blimp-1 is induced upon terminal differentiation of plasma cells. The transcriptional repressor activity of Blimp-1 has also been found to regulate T cell homeostasis and function, possibly by suppressing the expression of the cytokines IL-2 and interferon-γ during T cell development. More recent experiments have suggested that Blimp-1 also plays a major role in the formation of primordial germ cells (PGC) in developing mammalian embryos. In these experiments, Blimp-1-deficient mutant mouse embryos form a cluster of PGC-like cells which fail to show the expected migration, proliferation, and repression of homeobox genes that normally accompany specification of primordial germ cells.
Additional Names
Blimp-1 (CT), Presenilin enhancer protein 2, Gamma-secretase subunit PEN-2
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Description
Left: Western blot analysis of Blimp-1 in A549 cell lysate with Blimp-1 antibody at (A) 0.5 and (B) 1μg/ml.
Below: Immunofluorescence of Blimp-1 in mouse lung tissue with Blimp-1 antibody at 20 μg/ml.
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Other Product Images
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Source
PEN2 antibody was raised against a 13 amino acid peptide from near the carboxy terminus of human PEN2.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
Blimp-1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
Blimp-1 antibody can be used for detection of Blimp-1 by Western blot at 0.5 – 1 μg/ml.
Tested Application
E, WB, IF
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 3989P - Blimp-1 Peptide
Storage
Blimp-1 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1203 - A549 Cell Lysate
Species Reactivity
H, M, R
Protein GI Number
172072684
Protein Accession Number
NP_001189
Short Description
(CT) B-lymphocyte-induced maturation protein 1
This product belongs to the following categories:
Related Products
References- Turner CAJ, Mack DH, and Davis MM. Blimp-1, a novel zinc finger-containing protein that can drive the maturation of B-lymphocytes into immunoglobulin-secreting cells. Cell 1994; 77:297-306.
- Angelin-Duclos C, Cattoretti G, Lin K-I, et al. Commitment of B lymphocytes to a plasma cell fate is associated with Blimp-1 expression. J. Immunol. 2000; 165:5462-71.
- Reimold AM, Iwakoshi NN, Manis J, et al. Plasma cell differentiation requires the transcription factor XBP-1. Nature 2001; 412:300-7.
- Martins GA, Cimmino L, Shapiro-Shelef M, et al. Transcriptional repressor Blimp-1 regulates T cell homeostasis and function. Nature Immunol. 2006; 7:457-65.
Datasheet 08-02W
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