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Primary Antibodies
Bit1 Antibody
Background
Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment (1). Bit1 (Bcl-2 inhibitor of transcription 1) was recently identified as being involved in this process (2). Bit1 is a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis where it forms a complex with AES, a small Groucho/transducin-like enhancer of split (TLE) protein and induces caspase-independent apoptosis. Both AES and TLE proteins are transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination (3). It has been suggested that Bit1-AES complexes turn off a survival-promoting gene transcription program controlled by TLE (2). Interestingly, apoptosis of cells transfected with Bit1 and AES could be inhibited if the cells were allowed to attach to fibronectin through the α5β1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the α5β1 integrin (2)
Additional Names
Bit1 (NT), Bcl-2 inhibitor of transcription 1 / Peptidyl-tRNA hydrolase 2
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Description
Left: Western blot analysis of Bit1 in A-20 cell lysate with Bit1 antibody at (A) 1, (B) 2, and (C) 4 μg/ml.
Below: Immunocytochemistry of Bit1 in 3T3 cells with Bit1 antibody at 2 μg/ml.
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Other Product Images
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Source
Bit1 antibody was raised against a 15 amino acid peptide from near the amino-terminus of human Bit1.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
Bit1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human Bit1 / Bcl-2 inhibitor of transcription 1 / Peptidyl-tRNA hydrolase 2 (N-Terminus) Peptide (Cat. No. 3603P)
Application
Bit1 antibody can be used for the detection of Bit1 by Western blot at 1 – 2 μg/ml.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3603P - Bit1 Peptide
Storage
Bit1 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1288 - A-20 Cell Lysate
- Cat. No. 1212 - 3T3 Whole Cell Lysate
Species Reactivity
H, M, R
Protein GI Number
7706351
Protein Accession Number
NP_057161
Short Description
(NT) Bcl-2 inhibitor of transcription 1
This product belongs to the following categories:
Related Products
References- Martin SS and Vuori K. Regulation of Bcl-2 proteins during anoikis and amorphosis. Biochim Biophys Acta. 2004; 1692:145-57.
Datasheet 08-02W
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