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Primary Antibodies
Bim Antibody
Background
Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bim or BOD in human, mouse and rat (1,2). Bim/BOD interacts with diverse members in the pro-survival Bcl-2 sub-family including Bcl-2, Bcl-xL and Bcl-w. Bim/BOD induces apoptosis. The messenger RNA of Bim is ubiquitously expressed in multiple tissues and cell lines (1,2).
Additional Names
Bim (IN), BOD
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Description
Left: Western blot analysis of Bim in K562 (K) and A549 (A) whole cell lysates with Bim antibody at 1 μg/ml.
Below: Immunohistochemistry of Bim in human skin cancer cells with Bim antibody at 20 μg/ml.
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Other Product Images
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Source
Bim antibody was raised against a peptide corresponding to amino acids near the center of human Bim. The sequence is identical to that of mouse and differs from that of rat by one amino acid .
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
Bim antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human Bim (Intermediate Domain) Peptide (Cat. No. 2065P)
Application
Bim antibody can be used for detection of Bim by Western blot at 1 μg/ml dilution. A 23 kDa band can be detected.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 2065P - Bim Peptide
Storage
Bim antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1204 - K562 Cell Lysate
Species Reactivity
H, M, R
Protein GI Number
18202042
Protein Accession Number
O43521
Short Description
(IN) A new member in Bcl-2 family
This product belongs to the following categories:
Related Products
References- O'Connor L, Strasser A, O'Reilly LA, et al. Bim: a novel member of the Bcl-2 family that promotes apoptosis. EMBO J. 1998; 17:384-395.
- Hsu SY, Lin P, and Hsueh AJ BOD (Bcl-2-related ovarian death gene) is an ovarian BH3 domain-containing proapoptotic Bcl-2 protein capable of dimerization with diverse antiapoptotic Bcl-2 members. Mol. Endocrinol. 1998; 12:1432-40.
Datasheet 08-02W
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