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Primary Antibodies
Bik Antibody
Background
Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells and is caused by the activation of proteolytic enzymes termed caspases. Proteins that comprise the Bcl-2 family appear to control the activation of these enzymes. One such protein BIK was recently identified as an endoplasmic reticulum (ER)-residing pro-apoptotic member of the Bcl-2 homology domain-3 (BH3)-only group of the Bcl-2 family that stimulates mitochondrial release of cytochrome c following p53 induction of apoptosis. A significant fraction of BIK is found as an ER transmembrane protein, with most of the protein facing the cytosol. Restricting BIK to the ER membrane by replacing the transmembrane region with that of the ER-selective membrane anchor of cytochrome b(5) resulted in a decreased cytochrome c release from mitochondria and a corresponding drop in cell death. Recent evidence suggests that BIK cooperates with NOXA, another BH3-only protein, to somehow enhance the activation of Bax to stimulate the rapid release of cytochrome c from mitochondria.
Additional Names
Bik, Bcl-2-interacting killer, apoptosis inducer NBK
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Description
Left: Western blot analysis of BIK in Jurkat cell lysate with BIK antibody at (A) 1 and (B) 2 μg/ml.
Below: Immunocytochemistry of BIK in Jurkat cells with BIK antibody at 1 μg/ml.
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Other Product Images
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Source
BIK antibody was raised against a 15 amino acid peptide from near the amino terminus of human BIK.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
Bik antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
BIK antibody can be used for the detection of BIK by Western blot at 1 – 2 μg/ml.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 3819P - Bik Peptide
Storage
Bik antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1205 - Jurkat Cell Lysate
Species Reactivity
H, M
Protein GI Number
47678311
Protein Accession Number
CAG30276
Short Description
a member of the Bcl-2 family
This product belongs to the following categories:
Related Products
References- Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7.
- Germain M, Mathai JP, and Shore GC. BH-3-only BIK functions at the endoplasmic reticulum to stimulate cytochrome c release from mitochondria. J. Biol. Chem. 277:18053-60.
- Germain M, Mathai JP, McBride HM, et al. Endoplasmic reticulum BIK initiates DRP1-regulated remodelling of mitochondrial cristae during apoptosis. EMBO J. 2005; 24:1546-56.
Datasheet 08-02W
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