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Primary Antibodies
Bfl-1 Antibody
Background
Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells and is caused by caspase activation. Proteins that comprise the Bcl-2 family appear to control the activation of these enzymes. One such member is multi-domain antiapoptotic protein Bfl-1, which is overexpressed in stomach and other cancers. Bfl-1 can interact with Bax and suppress apoptosis by inhibiting the release of cytochrome c and caspase-3 activation. It is upregulated in cisplatin-resistant human bladder tumors, suggesting that its expression may be important for cisplatin resistance and inhibition of apoptosis in cancer cells. At least two isoforms of Bfl-1 are known to exist. Presumably due to post-translational modifications, Bfl-1 is often observed at higher molecular weight in SDS-PAGE than its predicted molecular weight would suggest.
Additional Names
Bfl-1 (NT), Bcl-2-related protein A1, Hemopoietic-specific early response protein
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Description
Left: Western blot analysis of Bfl-1 in mouse kidney tissue lysate with Bfl-1 antibody at (A) 1 and (B) 2 μg/ml.
Below: Immunohistochemistry of Bfl-1 in mouse kidney tissue with Bfl-1 antibody at 2 μg/ml.
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Other Product Images
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Source
Bfl-1 antibody was raised against a 14 amino acid peptide from near the amino terminus of human Bfl-1.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
Bfl-1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
Bfl-1 antibody can be used for the detection of Bfl-1 by Western blot at 1 – 2 μg/ml.
Tested Application
E, WB
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 3875P - Bfl-1 Peptide
Storage
Bfl-1 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1405 - Mouse Kidney Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
4757840
Protein Accession Number
NP_004040
Short Description
(NT) Bcl-2, apoptosis, cell death, Bax
This product belongs to the following categories:
Related Products
References- Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7.
- Choi SS, Park IC, Yun JW, et al. A novel Bcl-2 related gene, Bfl-1, is overexpressed in stomach cancer and preferentially expressed in bone marrow. Oncogene 1995; 11:1693-8.
- Kim JK, Kim KD, Lee E, et al. Up-regulation of Bfl-1/A1 via NF-κB activation in cisplatin-resistant human bladder cancer cell line. Cancer Lett. 2004; 212:61-70.
- Zhang H, Cowan-Jacob SW, Simonen M, et al. Structural basis of BFL-1 for its interaction with BAX and its anti-apoptotic action in mammalian and yeast cells. J. Biol. Chem. 2000; 275:11092-9.
Datasheet 08-02W
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