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Primary Antibodies
Beta-actin Antibody
Background
Actins are highly conserved proteins that are involved in cell motility, structure and integrity, processes that are crucial for tissue development and the development of organism (reviewed in 1). The actin cytoskeleton is one of the principal drivers of cell motility and is capable of responding to complex signaling cascades. Recent evidence suggests that it may play key roles in regulating apoptosis and aging (2). β actin is one of six different actin isoforms which have been identified. Like GAPDH, Β-actin is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.
Additional Names
Beta-actin (NT), Beta actin
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Description
Left: Western blot analysis of beta-actin in HeLa cell lysate with beta-actin antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.
Below: Immunocytochemistry of Actin in HeLa cells with Actin antibody at 1 μg/ml.
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Other Product Images
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Source
b-actin antibody was raised against a 16 amino acid peptide from near the amino-terminus of human b-actin.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
Beta-actin antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human b-actin / Beta actin (N-Terminus) Peptide (Cat. No. 3779P)
Application
b-actin antibody can be used for the detection of b-actin by Western blot at 1 – 2 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3779P - B-actin Peptide
Storage
Beta-actin antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1201 - HeLa Cell Lysate
Species Reactivity
H, M, R
Protein GI Number
12803203
Protein Accession Number
AAH02409
Short Description
(NT) a major cytoskeletal protein, N Terminus
This product belongs to the following categories:
Related Products
References- Lambrechts A, Van Troys, M and Ampe C. The actin cytoskeleton in normal and pathological cell motility. Int. J. Biochem. Cell Biol. 2004; 36:1890-909.
- Gourlay CW and Ayscough KR. The actin cytoskeleton: a key regulator of apoptosis and ageing. Nat. Rev. 2005; 6:583-9.
Datasheet 08-02W
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