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Primary Antibodies
Bcl-G Antibody
Background
Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain (reviewed in 1 and 2). BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bcl-G (3). The mRNA of Bcl-G encodes 2 isoforms, Bcl-GL, which is widely expressed in multiple tissues, and Bcl-GS, which is only found in testis. The Bcl-GS protein is predominantly localized to cytoplasmic organelles whereas Bcl-GL was distributed throughout the cytosol. Overexpression of either protein induced apoptosis, although Bcl-GS was far more potent than Bcl-GS. Apoptosis induction was dependent on the BH3 domain and could be suppressed by co-expression with the anti-apoptotic Bcl-XL protein (3)..
Additional Names
Bcl-G (CT), Bcl-2-like 14
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Description
Left: Western blot analysis of Bcl-G in U937 cell lysates with Bcl-G antibody at (A) 2.5 and (B) 5 μg/ml.
Below: Immunohistochemical staining of mouse testis tissue using Bcl-G antibody at 2 μg/ml.
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Other Product Images
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Source
Bcl-G antibody was raised against a peptide corresponding to 15 amino acids near the C-terminus of human Bcl-G.
Purification
Ion exchange chromatography purified
Clonality / Clone
This is a polyclonal antibody.
Host
Bcl-G antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Murine Bcl-G / Bcl-2-like 14 (C-Terminus) Peptide (Cat. No. 3165P)
Application
Bcl-G antibody can be used for detection of Bcl-G by Western blot at 2.5 to 5 μg/ml. Although antibody should react with both isoforms, only the Bcl-GS protein has been observed
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3165P - Bcl-G Peptide
Storage
Bcl-G antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1215 - U937 Cell Lysate
- Cat. No. 1416 - Mouse Testis Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
13540528
Protein Accession Number
NM_030766
Short Description
(CT) Bcl-2 family member
This product belongs to the following categories:
Related Products
References- Cory S, Huang DCS, and Adams JM. The Bcl-2 family: roles in cell survival and oncogenesis. Oncogene 2003; 22:8590-607.
- Heiser D, Labi V, Erlacher M, et al. The Bcl-2 protein family and its role in the development of neoplastic disease. Exp. Geron.. 2004; 39:1125-35.
- Guo B, Godzik A, and Reed JC. Bcl-G, a novel pro-apoptotic member of the Bcl-2 family. J. Biol. Chem. 2000; 276:2780-5.
Datasheet 08-02W
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