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Primary Antibodies
Bcl-2 Antibody
Background
Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer (reviewed in 1). Bcl-2 is the founding member of a family of over 20 proteins that are critical regulators of apoptosis (reviewed in 2 and 3). These can be divided into two classes: those that inhibit apoptosis and those that promote cell death. Bcl-2 is an inner mitochondrial membrane protein that inhibits apoptosis (4). It is thought to act by interacting with pro-apoptotic Bcl-2 family members such as Bak and Bad (5 for review). Overexpression of Bcl-2 has been linked to human cancers such as B-cell lymphoma and prostate cancer (4,6).
Additional Names
Bcl-2 (NT), Bcl-2
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Description
Left: Western blot analysis of Bcl-2 in Daudi cell lysates with Bcl-2 antibody at (A) 1, (B) 2, and (C) 4 μg/ml.
Below: Immunohistochemical staining of human kidney using Bcl-2 antibody at 2 μg/ml.
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Other Product Images
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Source
Bcl-2 antibody was raised against a peptide corresponding to 15 amino acids near the N-terminus of human Bcl-2.
Purification
Ion exchange chromatography purified
Clonality / Clone
This is a polyclonal antibody.
Host
Bcl-2 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Murine Bcl-2 (N-Terminus) Peptide (Cat. No. 3335P)
Application
Bcl-2 antibody can be used for detection of Bcl-2 by Western blot at 1 to 2 μg/ml. Bcl-2 antibody can also detect Bcl-2 by immunohistochemistry at 2 μg/ml. It is human and mouse reactive.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3335P - Bcl-2 Peptide
Storage
Bcl-2antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1224 - Daudi Cell Lysate
- Cat. No. 1305 - Human Kidney Tissue Lysate
Species Reactivity
H, M
Protein GI Number
20072668
Protein Accession Number
AAH27258
Short Description
(NT) a member of the bcl-2 family
This product belongs to the following categories:
Related Products
References- Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7.
- Cory S, Huang DCS, and Adams JM. The Bcl-2 family: roles in cell survival and oncogenesis. Oncogene 2003; 22:8590-607.
- Heiser D, Labi V, Erlacher M, et al. The Bcl-2 protein family and its role in the development of neoplastic disease. Exp. Geron. 2004; 39:1125-35.
- Hockenbery D, Nunez G, Milliman C, et al. Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death. Nature 1990; 348:334-6.
Datasheet 08-02W
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