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Primary Antibodies
Bcl-10 Antibody
Background
Apoptosis is related to many diseases including cancer. Cell death signals are transduced by death domain (DD) and caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. CARD containing cell death regulators include ARC, RAIDD, Apaf-1, caspase-9, and caspase-2. A novel CARD containing protein was recently identified by several groups and designated Bcl10, CIPER, mE10, CARMEN, CLAP. Bcl10 is a cellular homolog of the equine herpesvirus-2 E-10 gene. Overexpression of Bcl10 induces JNK, p38, and NF-κB activation. Bcl10 interacts with caspase-9 and enhances pro-caspase-9 processing (3) and induces apoptosis through caspase-9 activation . Bcl10 exhibits a variety of mutations in MALT lymphomas and in B and T cell lineage lymphomas indicating that it may be commonly involved in the pathogenesis of human malignancy . Bcl10 is expressed in many human and murine tissues and cell lines.
Additional Names
Bcl-10 (NT), CIPER, CLAP
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Description
Left: Western blot analysis of Bcl-10 in Raji whole cell lysate in the absence (A) or presence (B) of peptide (2161P) with Bcl-10 antibody at 1:500 dilution.
Below: Immunocytochemistry of Bcl10 in Raji cells with Bcl10 antibody at 1 μg/ml.
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Other Product Images
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Source
Bcl10 antibody was raised against a peptide corresponding to amino acids near the amino terminus of human Bcl10.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
Bcl-10 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Murine BCL-10 (N-Terminus) Peptide (Cat. No. 2161P)
Application
Bcl-10 antibody can be used for detection of BCL10 by Western blot at 1:500 dilution. An approximately 31 kDa band can be detected.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 2161P - Bcl-10 Peptide
Storage
Bcl-10 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1207 - Raji Cell Lysate
Species Reactivity
H, M, R
Protein GI Number
5070371
Protein Accession Number
AF134395
Short Description
(NT) Involved in apoptosis and tumor
This product belongs to the following categories:
Related Products
References- Willis TG, Jadayel DM, Du MQ, et al. Bcl10 is involved in t(1;14)(p22;q32) of MALT B cell lymphoma and mutated in multiple tumor types. Cell 1999;96(1):35-45
- Koseki T, Inohara N, Chen S, et al. CIPER, a novel NF κB-activating protein containing a caspase recruitment domain with homology to Herpesvirus-2 protein E10. J Biol Chem 1999;274(15):9955-61
- Yan M, Lee J, Schilbach S, Goddard A, Dixit V. mE10, a novel caspase recruitment domain-containing proapoptotic molecule. J Biol Chem 1999;274(15):10287-92
- Thome M, Martinon F, Hofmann K, et al. Equine herpesvirus-2 E10 gene product, but not its cellular homologue, activates NF-κB transcription factor and c-Jun N-terminal kinase. J Biol Chem 1999;274(15):9962-8
Datasheet 08-02W
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