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Primary Antibodies
Bak Antibody
Background
Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer (reviewed in 1). The Bcl-2 family of proteins is comprised of critical regulators of apoptosis that can be divided into two classes: those that inhibit apoptosis and those that promote cell death (reviewed in 2 and 3). Bak, a pro-apoptotic Bcl-2 family member, is an oligomeric protein that localizes to the mitochondria (4,5). It is thought to share significant functional homology with Bax, another pro-apoptotic Bcl-2 family member, as disruption of bak or bax has little effect on cell death, but mice lacking both genes display multiple developmental defects and cells lacking bak and bax show decreased apoptotic capability (6,7).
Additional Names
Bak (NT), Bak
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Description
Left: Western blot analysis of Bak in L1210 cell lysates with Bak antibody at (A) 1 and (B) 2 μg/ml.
Below: Immunocytochemistry of Bak in L1210 cells with Bak antibody at 2 μg/ml.
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Other Product Images
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Source
Bak antibody was raised against a peptide corresponding to 13 amino acids near the amino-terminus of human Bak.
Purification
Ion exchange chromatography purified
Clonality / Clone
This is a polyclonal antibody.
Host
Bak antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human Bak (N-Terminus) Peptide (Cat. No. 3347P)
Application
Bak antibody can be used for detection of Bak by Western blot at 1 to 2 μg/ml.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3347P - Bak Peptide
Storage
Bak antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1284 - L1210 Cell Lysate
Species Reactivity
H, M
Protein GI Number
2493274
Protein Accession Number
Q16611
Short Description
(NT) a member of the bcl-2 family
This product belongs to the following categories:
Related Products
References- Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7.
- Cory S, Huang DCS, and Adams JM. The Bcl-2 family: roles in cell survival and oncogenesis. Oncogene 2003; 22:8590-607.
- Heiser D, Labi V, Erlacher M, et al. The Bcl-2 protein family and its role in the development of neoplastic disease. Exp. Geron. 2004; 39:1125-35.
- Kiefer M, Brauer MJ, Powers VC, et al. Modulation of apoptosis by the widely distributed Bcl-2 homologue Bak. Nature 1995; 374:736-9.
Datasheet 08-02W
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