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Primary Antibodies
BTLA Antibody
Background
BTLA Antibody. This antibody is expected to recognize both reported isoforms (NP_861445.3; NP_001078826.1).
Additional Names
BTLA, B and T lymphocyte associated, BTLA1, CD272, FLJ16065, MGC129743, B and T lymphocyte attenuator
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Description
Left: Western blot analysis of BTLA in Human Placenta lysate (35μg protein in RIPA buffer) using BTLA antibody (0.03μg/ml).
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Source
BTLA antibody was raised against a synthetic peptide of BTLA.
Purification
BTLA antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality / Clone
This is a polyclonal antibody.
Host
BTLA antibody was raised in goat.
Please use anti-goat secondary antibodies.
Application
Peptide ELISA: Antibody detection limit dilution 1:4,000. Western Blot: Approx. 35kDa band observed in Mouse Spleen lysates (calculated MW of 32.8kDa according to Human NP_861445.3 and of 34.2kDa according to Mouse NP_001032808.2). Recommended concentration: 1-3μg/ml.
Tested Application
E, WB
Buffer
BTLA antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Blocking Peptide
Cat. No. 46-766P - BTLA Peptide
Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Positive Control
- Cat. No. 1309 - Human Placenta Lysate
Species Reactivity
H, M, R
Protein Accession Number
NP_861445.3, NP_001078826.1
Short Description
BTLA
This product belongs to the following categories:
Related Products
References- Lasaro MO, Tatsis N, Hensley SE, Whitbeck JC, Lin SW, Rux JJ, Wherry EJ, Cohen GH, Eisenberg RJ, Ertl HC. Targeting of antigen to the herpesvirus entry mediator augments primary adaptive immune responses. Nat Med. 2008 Feb;14(2):205-12. Epub 2008 Jan 13.
Datasheet 09-01W
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