|
Primary Antibodies
BTK Antibody
Background
Bruton’s tyrosine kinase (BTK) was initially identified as a member of the src family for protein-tyrosine kinases that was involved in X-linked aγ-globulinaemia (1), and has since been shown to involved in a number of signaling pathways in hemapoietic lineage (reviewed in 2). It has recently been shown to interact with members of the toll-like receptor (TLR) family such as TLR4, 6, 8, and 9 (3). The TLRs are critical molecules in both the innate and adaptive immunity and can recognize diverse microbial pathogens (reviewed in 4). BTK has also been shown to interact with key proteins involved in TLR4 signal transduction such as MyD88, TIRAP, and IRAK, but not TRAF-6, suggesting that BTK is involved in lipopolysaccharide signal transduction (3).
Additional Names
BTK (NT), ATK, BPK
|
Description
Left: Western blot analysis of BTK in U937 cell lysate with BTK antibody at (A) 0.5, (B) 1, and (C) 2 μg/ml.
Below: Immunocytochemistry of BTK in Daudi cells with BTK antibody at 10 μg/ml.
|
|
Other Product Images
|
Source
BTK antibody was raised against a synthetic peptide corresponding to 16 amino acids near the amino-terminus of human BTK.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
BTK antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human BTK / ATK / BPK (N-Terminus) Peptide (Cat. No. 3395P)
Application
BTK antibody can be used for the detection of BTK by Western blot at 0.5 to 1 μg/ml.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3395P - BTK Peptide
Storage
BTK antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1215 - U937 Cell Lysate
- Cat. No. 1224 - Daudi Cell Lysate
Species Reactivity
H, M
Protein GI Number
547759
Protein Accession Number
Q06187
Short Description
(NT) Bruton's Tyrosine Kinase
This product belongs to the following categories:
Related Products
References- Vetrie D, Vorechovsky I, Sideras P, et al. The gene involved in X-linked aγglobulinaemia is a member of the src family of tyrosine kinases. Nature 1993; 361:226-33.
- Kawakami Y, Kitaura J, Hata D, et al. Functions of Bruton's tyrosine kinase in mast and B cells. J. Leukoc. Biol. 1999; 65(3):286-90.
- Jefferies CA, Doyla S, Brunner C, et al. Bruton’s tyrosine kinase is a Toll/Interleukin-1 receptor domain-binding protein that participates in nuclear factor kB activation by Toll-like receptor 4. J. Biol. Chem. 2003; 278:26258-26264.
- Akira S and Takeda K. Toll-like receptor Signalling. Nat. Rev. Immunol. 2004; 4:499-511.
Datasheet 08-02W
|
|
