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Primary Antibodies
BRAL1 Antibody
Background
BRAL1 is a member a superfamily consisting of several highly homologous hyaluronan and proteoglycan binding link proteins. BRAL1 is predominantly expressed in brain tissue and spinal cord. Like other members in the link-module superfamily, BRAL1 contains an immunoglobulin-like fold and two proteoglycan tandem repeats (PTRs). Its mRNA expression pattern is similar to other lectican proteoglycans, suggesting that BRAL1 may act to stabilize the binding between the extracellular matrix molecule hyaluronan and brevican. Immunostaining of mouse brain showed BRAL1 expression at P20 in the white matter of the developing cerebellum and in myelinated fiber tracts in the adult brain, suggesting that expression starts when axonal myelination occurs.
Additional Names
BRAL1, Brain link protein-1, hyaluronan and proteoglycan link protein 2, HAPLN2
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Description
Left: Western blot analysis of BRAL1 in human brain tissue lysate with BRAL1 antibody at (A) 1 and (B) 2 μg/ml.
Below: Immunohistochemical staining of human brain tissue using BRAL1 antibody at 2.5 μg/ml.
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Other Product Images
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Source
BRAL1 antibody was raised against a 12 amino acid peptide from near the center of human BRAL1.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
BRAL1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
BRAL1 antibody can be used for the detection of BRAL1 by Western blot at 1 – 2 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 4501P - BRAL1 Peptide
Storage
BRAL1 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1303 - Human Brain Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
11141887
Protein Accession Number
NP_068589
Short Description
Brain-link protein 1
This product belongs to the following categories:
Related Products
References- Spicer AP, Joo A, and Bowling Jr. RA. A hyaluronan binding link protein gene family whose members are physically linked adjacent to chrondroitin sulfate proteoglycan core protein genes. J. Biol. Chem. 2003; 278:21083-91.
- Hirakawa S, Oohashi T, Su W-D, et al. The brain link protein-1 (BRAL1): cDNA cloning, genomic structure, and characterization as a novel link protein expressed in adult brain. Biochem. Biophys. Res. Comm. 2000; 276:982-9.
- Oohashi T, Hirakawa S, Bekku Y, et al. Bral1, a brain-specific link protein, colocalizing with the versican V2 isoform at the nodes of Ranvier in developing and adult mouse central nervous systems. Mol. Cell Neurosci. 2002; 19:43-57.
Datasheet 08-02W
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