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Primary Antibodies
BID Antibody
Background
BID Antibody. This antibody is expected to recognize all three reported isoforms (as represented by NP_932070.1; NP_001187.1; NP_932071.1)
Additional Names
BID, BH3 interacting domain death agonist , HGNC:1050, MGC15319, MGC42355 , BH3-interacting domain death agonist, Human BID coding sequence, apoptic death agonist
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Description
Left: Western blot analysis of BID in A431 lysate (35μg protein in RIPA buffer) using BID antibody (0.1μg/mL).
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Source
BID antibody was raised against a synthetic peptide near the C-terminus of BID.
Purification
BID antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality / Clone
This is a polyclonal antibody.
Host
BID antibody was raised in goat.
Please use anti-goat secondary antibodies.
Application
Peptide ELISA: Antibody detection limit dilution 1:8,000. Western Blot: Approx 26+24kDa bands observed in human fibroblast A431 lysates (calculated MW of 26.8kDa according to NP_932070.1 and 22.0kDa according to NP_001187.1). Recommended for use at 1-3μg/ml.
Tested Application
E, WB
Buffer
BID antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Blocking Peptide
Cat. No. 45-021P - BID Peptide
Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Positive Control
- Cat. No. 1202 - A431 Cell Lysate
Species Reactivity
H
Protein Accession Number
NP_932070.1, NP_001187.1, NP_932071.1
Short Description
(CT) BID
This product belongs to the following categories:
Related Products
References- Lund T, Stokke T, Olsen OE, Fodstad O. Garlic arrests MDA-MB-435 cancer cells in mitosis, phosphorylates the proapoptotic BH3-only protein Bim(EL) and induces apoptosis. Br J Cancer. 2005 Apr 12; [Epub ahead of print]
Datasheet 08-02W
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