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Detection Sets
BH3-Only Protein Detection Set
Background
Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. This process is regulated by the interplay of pro- and anti-apoptotic members of the B-cell lymphoma 2 (Bcl-2) family. The BH3-only (Bcl-2-homology domain 3 only) proteins are a pro-apoptotic subgroup of the Bcl-2 family that are critical initiators of lymphocyte apoptosis and other processes. Unlike the other members of the Bcl-2 family which contain at least two Bcl-2 homology (BH) domains, these proteins contain just one, the BH3 domain. Some of the members of this family, such as Hrk, may show little sequence homology to the others, but all BH-only proteins can bind to at least some pro-survival Bcl-2-like proteins through this BH3 domain and trigger apoptosis when overexpressed. For example, the proteins Bad, Bim and Bmf regulate apoptosis by forming heterodimers with the anti-apoptotic proteins Bcl-2 and Bcl-xL. Bim, Noxa, and PUMA are activated by p53 following DNA damage although the exact mechanisms are not entirely clear. Noxa may also be involved with the activation of Bik, a BH3-only protein whose localization in the endoplasmic reticulum (ER) suggests that Bik may play a role in ER-stress-induced apoptosis. While full-length Bid also promotes apoptosis when expressed, its activity is enhanced following cleavage by enzymes such as capsase-8, suggesting that Bid may play a key role in death-receptor induced apoptosis.
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Description
For images please see PDF data sheet
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Kit Contents
Bad Antibody, Catalog No. 3343 (50μg)
Bid Antibody (CT), Catalog No. 3353 (50μg)
Bik Antibody, Catalog No. 3819 (50μg)
Bim Antibody (IN), Catalog No. 2065 (50μg)
Bmf Antibody (NT), Catalog No. 3031 (50μg)
Hrk Antibody, Catalog No. 3771 (50μg)
Noxa Antibody, Catalog No. 2437 (50μg)
PUMA Antibody (CT), Catalog No. 3041 (50μg)
Source
Rabbit polyclonal antibodies were raised against peptides corresponding to amino acid sequences from each of the corresponding proteins.
Application
These polyclonal antibodies can be used for detection of Bad, Bid, Bik, Bim, Bmf, Hrk, Noxa and PUMA by immunoblot at 1 – 2 μg/ml and can detect their respective proteins via immunohistochemistry at 1 – 10 μg/ml.
Tested Application
WB, IHC
Blocking Peptide
Bad Peptide (CT), Catalog No. 343P
Bid Peptide (CT), Catalog No. 3353P
Bik Peptide, Catalog No. 38129P
Bim Peptide, Catalog No. 2065P
Bmf Peptide (CT), Catalog No. 3031P
Hrk Peptide, Catalog No. 3771P
Noxa Peptide, Catalog No. 2437P
PUMA Peptide (CT), Catalog No. 3041P
Bcl-2 Antibody (NT), Catalog No. 3335
Caspase-8 Antibody (CT), Catalog No. 3473
Storage
Antibodies are supplied as affinity chromatography purified IgG, in PBS containing 0.02% sodium azide. Antibodies should be stored at -20˚C.
Positive Control
- Bad Antibody: T24 Cell Lysate, Catalog No. 1213
Bid Antibody: Mouse Lung Tissue Lysate, Catalog No. 1402
Bik Antibody: Jurkat Cell Lysate, Catalog No. 1205
Bim Antibody: K562 Cell Lysate, Catalog No. 1204
Bmf Antibody: HepG2 Cell Lysate, Catalog No. 1211
Hrk Antibody: Mouse Pancreas Tissue Lysate, Catalog No. 1414
Noxa Antibody: Human Stomach Tissue Lysate, Catalog No. 1319
PUMA Antibody: K562 Cell Lysate, Catalog No. 1204
Species Reactivity
see datasheet
Short Description
Bad, Bid, Bik, Bim, Bmf, Hrk, Noxa and PUMA
This product belongs to the following categories:
Related Products
References- Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7.
- Cory S, Huang DCS, and Adams JM. The Bcl-2 family: roles in cell survival and oncogenesis. Oncogene 2003; 22:8590-607.
- Strasser A. The role of BH3-only proteins in the immune systems. Nat. Rev. 2005
- Inohara N, Ding L, Chen S, et al. harakiri, a novel regulator of cell death, encodes a protein that activates apoptosis and interacts selectively with survival-promoting proteins Bcl-2 and Bcl-XL. EMBO J. 1997; 16:1686-94.
Datasheet 0803-W
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