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Primary Antibodies
BDNF Antibody
Background
BDNF Biotin Conjugated Antibody
Additional Names
Abrineurin, Brain Derived Neurotrophic Factor, MGC34632, Neurotrophin
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Description
Brain derived neurotrophic factor (BDNF) is a member of the neurotrophin family of growth factors that includes NGF, NT3, and NT4. All neurotrophins have six conserved cysteine residues and share a 55% sequence identity at the amino acid level. BDNF is a potent neurotrophic factor that supports the growth and survivability of nerve and/or glial cells. BDNF has been shown to enhance the survival and differentiation of several classes of neurons in vitro, including neural crest and placode derived sensory neurons, dopaminergic neurons in the substantia nigra, basal forebrain cholinergic neurons, hippocampal neurons, and retinal ganglial cells. BDNF is expressed within peripheral ganglia and is not restricted to neuronal target fields, raising the possibility that BDNF has paracrine or even autocrine actions on neurons as well as non neuronal cells. Expression of BDNF is reduced in both Alzheimer's and Huntington disease patients. In addition, functional studies showed that age-associated changes in BDNF-mediated pathways can enhance inflammation and increase myocardial injury after myocardial infarction in the aging heart.
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Source
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hBDNF (human BDNF).
Conjugate
Biotin
Purification
Anti-hBDNF specific antibody was purified by affinity chromatography and then biotinylated.
Clonality / Clone
This is a polyclonal antibody.
Host
BDNF antibody was raised in rabbit.
Please use rabbit secondary antibodies.
Preparation
Lyophilized from sterile-filtered PBS solution.
Application
ELISA:
To detect hBDNF by direct ELISA (using 100 μl/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 μg/ml. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hBDNF.
Western Blot:
To detect hBDNF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBDNF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Tested Application
E, WB
Storage
BDNF lyophilized antibody is stable for at least 6 months when kept at -20˚C. The biotinylated antibody should be reconstituted to a concentration of 0.1 - 1.0 mg/ml with sterile PBS solution containing 0.1% BSA. This solution can be stored at 4˚C for at least one month without detectable loss of activity. Frozen aliquots of this solution are stable for at least 6 months when kept at -20˚C. Avoid more than one freeze-thaw cycle.
Species Reactivity
H
Short Description
Biotin - Anti-Human BDNF - Rabbit
This product belongs to the following categories:
Related Products
Datasheet 1001-W
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