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Primary Antibodies
BCL7A Antibody
Background
BCL7A Antibody.
Additional Names
BCL7A, BCL7, B-cell CLL/lymphoma 7A, B-cell CLL/lymphoma-7
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Description
Left: Western blot analysis of BCL7A in HeLa lysate (RIPA buffer, 35μg total protein per lane) using BCL7A antibody (0.5μg/mL).
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Source
BCL7A antibody was raised against a synthetic peptide of BCL7A.
Purification
BCL7A antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality / Clone
This is a polyclonal antibody.
Host
BCL7A antibody was raised in goat.
Please use anti-goat secondary antibodies.
Application
Western blot: Approx 35kDa band seen in HeLa cell lysates. Recommended for use at 0.5-2μg/ml. Please note that BCL7A, according to NP_066273, has several consensus glycosylation sites. Glycosylation can affect migration of proteins on SDS-PAGE [Hames, B.D and Rickwood, D., eds. Gel electrophoresis of proteins: a practical approach. Oxford University Press]. This may be the reason why we see an approx 35kDa band rather than the predicted 26kDa band.
Tested Application
E, WB
Buffer
BCL7A antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Blocking Peptide
Cat. No. 45-325P - BCL7A Peptide
Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Positive Control
- Cat. No. 1201 - HeLa Cell Lysate
Species Reactivity
H, M
Protein Accession Number
NP_066273
Short Description
BCL7A
This product belongs to the following categories:
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References- Zani VJ, Asou N, Jadayel D, Heward JM, Shipley J, Nacheva E, Takasuki K, Catovsky D, Dyer MJ. Molecular cloning of complex chromosomal translocation t(8;14;12)(q24.1;q32.3;q24.1) in a Burkitt lymphoma cell line defines a new gene (BCL7A) with homology to caldesmon. Blood 1996 Apr 15;87(8):3124-34
Datasheet 08-01W
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