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Primary Antibodies
BAP31 Antibody
Background
Bap31 and the related protein Bap29 are endoplasmic reticulum (ER) and ER-vesicle membrane proteins and members of the B-cell receptor-associated protein family (1,2). These two proteins are highly homologous and can form homo- and heterodimers. Bap31 is thought to be involved in the intracellular trafficking of several molecules such as MHC Class I molecules and CD11b/CD18 (3,4). It may also play a role in the initiation of ER stress-induced apoptosis through its association with caspase-8 via a death effector domain in its cytoplasmic tail, possibly through the promotion of membrane fragmentation and the release of cytochrome c from mitochondria (5). Bap31 itself contains two caspase cleavage sites and is cleaved during apoptosis. The p20 fragment of Bap31, when expressed ectopicially, is also a potent inducer cell death (5).
Additional Names
BAP31, B cell receptor-associated protein 31 BCR associated protein 31
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Description
Left: Western blot analysis of BAP31 in Ramos cell lysate with BAP31 antibody at (A) 0.5, (B) 1 and (C) 2μg/ml.
Below: Immunohistochemistry of BAP31 in rat lung tissue with BAP31 antibody at 2 μg/ml.
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Other Product Images
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Source
BAP31 antibody was raised against a 17 amino acid peptide from near the carboxy terminus of human BAP31.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
BAP31 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human BAP31 (C-Terminus) Peptide (Cat. No. 3665P)
Application
BAP31 antibody can be used for the detection of BAP31 by Western blot at 0.5 – 1 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3665P - BAP31 Peptide
Storage
BAP31 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1225 - Ramos Cell Lysate
- Cat. No. 1462 - Rat Lung Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
32171186
Protein Accession Number
NP_005736
Short Description
A novel B-cell receptor-associated protein family member
This product belongs to the following categories:
Related Products
References- Ng F, Nguyen M, Kwan T, et al. p28 BAP31, a Bcl-2/Bcl-XL- and procaspase-8-associated proteinin the endoplasmic reticulum. J. Cell Biol. 1997; 139:327-38.
- Kim KM, Adachi T, Nielsen PJ, et al. Two new proteins preferentially associated with membrane immunoglobulin D. EMBO J. 1994; 13:3793-800.
- Paquet M-E, Cohen-Doyle M, Shore GC, et al. Bap29/31 influences the intracellular traffic of MHC class I molecules. J. Immunol. 2004; 172:7548-55.
- Zen K, Utech M, Liu Y, et al. Association of BAP31 with CD11b/CD18. Potential role in intracellular trafficking of CD11b/CD18 in neutrophils. J. Biol. Chem. 2004; 279:44924-30.
Datasheet 08-02W
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