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Primary Antibodies
BAP3 Antibody
Background
BAP3 was initially identified through interaction in a yeast two-hybrid system with the brain-specific angiogenesis inhibitor 1, a p53-target gene that encodes a seven-span transmembrane protein member of the secretin receptor family. BAP3 is predominantly expressed in the brain and possess high homology with Munc13 and synaptotagmin, suggesting that BAP3 may play a role in regulating neurotransmitter release. Recent experiments have shown that BAP3 is induced in certain tumors such as desmoplastic small round cell tumor. Ectopic expression of BAP3 in tumor cells dramatically enhances growth in low serum conditions and colony formation in soft agar, suggesting that the regulated exocytotic pathway may play a role in cancer cell proliferation. BAP3 is known to have two isoforms; this BAP3 antibody will recognize only isoform 2. Lower molecular weight bands may represent cleavage or degradation products.
Additional Names
BAP3 (NT2), BAI1-associated protein 3, BAIAP3
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Description
Left: Western blot analysis of BAP3 in SK-N-SH cell lysate with BAP3 antibody at (A) 1 and (B) 2 μg/ml.
Below: Immunohistochemical staining of human brain tissue using BAP3 antibody at 5 μg/ml.
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Other Product Images
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Source
BAP3 antibody was raised against a 13 amino acid peptide from near the amino terminus of human BAP3.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
BAP3 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
BAP3 antibody can be used for the detection of BAP3 by Western blot at 2 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 4505P - BAP3 Peptide
Storage
BAP3 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1220 - SK-N-SH Cell Lysate
Species Reactivity
H
Protein GI Number
119606077
Protein Accession Number
EAW85671
Short Description
(NT2) BAI1-associated protein 3
This product belongs to the following categories:
Related Products
References- Shiratsuchi T, Oda K, Nishimori H, et al. Cloning and characterization of BAP (BAI-associated protein 3), a C2 domain-containing protein that interacts with BAI1. Biochem. Biophys. Res. Comm. 1998; 251:158-65.
- Palmer RE, Lee SB, Wong JC, et al. Induction of BAIAP3 by the EWS-WT1 chimeric fusion implicates regulated exocytosis in tumorigenesis. Cancer Cell 2002; 2:497-505.
Datasheet 08-02W
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