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Primary Antibodies
BAP29 Antibody
Background
Bap29 and the related protein Bap31 are endoplasmic reticulum (ER) and ER-vesicle membrane proteins and members of the B-cell receptor-associated protein family (1,2). These two proteins are highly homologous and can form homo- and heterodimers. Both Bap29 and Bap31 interact with membrane-bound immunoglobulins (mIgs), such as IgM and IgD, which with Ig-α/Ig-β heterodimers form B cell antigen receptors (3). Binding of the Bap29/Bap31 heterodimer correlates with the ER retention of non-Ig-α/Ig-β bound mIg complexes, suggesting that Bap29 and Bap31 may act as chaperones transmembrane regions of various proteins (3). Bap29 possesses multiple isoforms.
Additional Names
BAP29 (IN), B cell receptor-associated protein 29, BCR associated protein 29
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Description
Left: Western blot analysis of Bap29 in human heart tissue lysate with Bap29 antibody at (A) 0.5, (B) 1 and (C) 2μg/ml.
Below: Immunohistochemistry of BAP29 in human heart tissue with BAP29 antibody at 10 μg/ml.
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Other Product Images
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Source
BAP29 antibody was raised against a 17 amino acid peptide from near the center of human Bap29 Bap29 antibody is specific for isoform a of Bap29 .
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
BAP29 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human BAP29 (Intermediate Domain) Peptide (Cat. No. 3667P)
Application
Bap29 antibody can be used for the detection of Bap29 by Western blot at 0.5 – 1 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3667P - BAP29 Peptide
Storage
BAP29 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1301 - Human Heart Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
56549093
Protein Accession Number
NP_001008405
Short Description
(IN) A novel B-cell receptor-associated protein family member
This product belongs to the following categories:
Related Products
References- Kim KM, Adachi T, Nielsen PJ, et al. Two new proteins preferentially associated with membrane immunoglobulin D. EMBO J. 1994; 13:3793-800.
- Ng F, Nguyen M, Kwan T, et al. p28 BAP31, a Bcl-2/Bcl-XL- and procaspase-8-associated protein the endoplasmic reticulum. J. Cell Biol. 1997; 139:327-38.
- Schamel WW, Kuppig S, Becker B, et al. A high-molecular-weight complex of membrane proteins BAP29/BAP31 is involved in the retention of membrane-bound IgD in the endoplasmic reticulum. Proc. Natl. Acad. Sci. USA 2003; 100:9861-6.
Datasheet 08-02W
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