|
Primary Antibodies
BANF1 Antibody
Background
Barrier-to-autointegration factor 1 (BANF1) is a conserved chromatin protein that non-specifically binds double-stranded DNA. BAF also interacts with a family of nuclear proteins that include LAP2, emerin, and MAN1. It is also a host cell component of retroviral pre-integration complexes (PICs), including that of HIV. BAF will bind to p55 Gag (the structural precursor of HIV-1 virions) as well as its cleaved product matrix. In addition to being a host cell component of the PIC, it is thought that BAF is also present at low levels in incoming virions, and thus might contribute to the assembly or activity of HIV-1 PICs through direct binding to matrix as well as DNA. Despite its predicted molecular weight, BANF1 often migrates at a higher size in SDS-PAGE.
Additional Names
BANF1 (NT), Barrier to autointegration factor 1, BAF, breakpoint cluster region protein 1
|
Description
Left: Western blot analysis of BANF1 in mouse kidney tissue lysate with BANF1 antibody at (A) 0.5, (B) 1 and (C) 2 μg/ml.
Below: Immunofluorescence of BANF1 in human kidney tissue with BANF1 antibody at 20 μg/ml.
|
|
Other Product Images
|
Source
BANF1 antibody was raised against a 19 amino acid peptide from near the amino terminus of human BANF1.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
BANF1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
BANF1 antibody can be used for detection of BANF1 by Western blot at 0.5 – 1 μg/ml.
Tested Application
E, WB, IF
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 4019P - BANF1 Peptide
Storage
BANF1 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1405 - Mouse Kidney Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
13543577
Protein Accession Number
AAH05942
Short Description
(NT) Barrier to autointegration factor
This product belongs to the following categories:
Related Products
References- Furukawa K. LAP2 binding protein 1 (L2BP1/BAF) is a candidate mediator of LAP2-chromatin interaction. J. Cell Sci. 1999; 112:2485-92.
- Cai M, Huang Y, Ghirlando R, et al. Solution structure of the constant region of nuclear envelope protein LAP2 reveals two LEM-domain structures: one binds BAF and the other binds DNA. EMBO J. 2001; 20:4399-407.
- Chen H and Engelman A. The barrier-to-autointegration protein is a host factor for HIV type 1 integration. Proc. Natl. Acad. Sci. USA 1998; 95:15270-4.
Datasheet 08-02W
|
|
