|
Detection Sets
BAFF and Receptor Detection Set
Background
Members in the TNF superfamily regulate immune responses and induce apoptosis. Two novel members were recently identified by several groups and designated BAFF and APRIL. Both proteins stimulate B cell proliferation, humoral immune responses, NF-κB activation, and apoptosis. Two receptors, TACI and BCMA, recognize both BAFF and APRIL; a third receptor, BAFF-R, is specific for BAFF. These ligands and their receptors are involved in the development of autoimmune diseases as well as cancer.
|
Description
For images please see PDF data sheet
|
Kit Contents
BAFF Antibody, Cat. No.. 2221 (50μg)
APRIL Antibody, Cat. No.. 2415 (50μg)
TACI Antibody, Cat. No.. 2395 (50μg)
BCMA Antibody, Cat. No.. 2397 (50μg)
BAFF-R Antibody, Cat. No.. 3097 (50μg)
Source
Rabbit polyclonal antibodies were raised against peptides corresponding to amino acid sequences from each of the corresponding proteins.
Application
These polyclonal antibodies can be used for detection of BAFF and its receptors by Western blot at 2 μg/ml to 1 μg/ml and Immunohistochemistry. Whole cell lysate from HepG2 cells can be used as a positive control for each antibody except BAFF-R antibody; murine spleen lysate can be used as a positive control for that. For research use only.
Tested Application
WB, IHC
Blocking Peptide
BAFF Peptide, Cat. No.. 2221P
APRIL Peptide, Cat. No.. 2415P
TACI Peptide, Cat. No.. 2395P
BCMA Peptide, Cat. No.. 2397P
BAFF-R Peptide, Cat. No.. 3097P
Storage
Antibodies are supplied as affinity chromatography purified IgG, in PBS containing 0.02% sodium azide. Antibodies should be stored at -4˚C.
Positive Control
- HepG2 Lysate, Cat. No. 1211
- Murine Spleen Lysate, Cat. No. 1406.
Species Reactivity
see datasheet
Short Description
BAFF, APRIL, TACI, BCMA, and BAFF-R
This product belongs to the following categories:
Related Products
References- Moore PA, Belvedere O, Orr A, et al. BLyS: member of the tumor necrosis factor family and B lymphocyte stimulator. >Science 1999; 285:260-3.
- Schneider P, MacKay F, Steiner V, et al. BAFF, a novel ligand of the tumor necrosis factor family, stimulates B cell growth. J Exp Med 1999; 189:1747-56.
- Shu HB, Hu WH, Johnson H. TALL-1 is a novel member of the TNF family that is down-regulated by mitogens. J Leuk Biol 1999; 65:680-3.
- Hahne M, Kataoka T, Schroter M, et al. APRIL, a new ligand of the tumor necrosis factor family, stimulates tumor cell growth. J Exp Med. 1998; 188(6):1185-90.
Datasheet 0803-W
|
|
