|
Primary Antibodies
BAD Antibody
Background
Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death (reviewed in 1 and 2). Bcl-2 homology 3 (BH3) domain containing pro-apoptotic proteins, such as Bax, Bid, and Bik, form a growing subclass of the Bcl-2 family. Another such protein is the Bcl-2-antagonist of cell death (Bad). Bad regulates apoptosis by forming heterodimers with anti-apoptotic proteins Bcl-2 and Bcl-xL, thereby preventing them from binding with Bax (3). Bad activity is regulated by its phosphorylation; it is inactivated by kinases such as Akt and MAP kinase and thus promotes cell survival (4), whereas JNK-induced phosphorylation promotes the apoptotic role of Bad (5).
Additional Names
BAD, Bcl-2-like 8
|
Description
Left: Western blot analysis of Bad in T24 cell lysates with Bad antibody at (A) 0.5, (B) 1, and (C) 2 μg/ml.
Below: Immunohistochemical staining of rat thymus using Bad at 2 μg/ml.
|
|
Other Product Images
|
Source
Bad antibody was raised against a peptide corresponding to 15 amino acids near the C-terminus of human Bad.
Purification
Ion exchange chromatography purified
Clonality / Clone
This is a polyclonal antibody.
Host
BAD antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Murine Bad / Bcl-2-like 8 Peptide (Cat. No. 3343P)
Application
Bad antibody can be used for detection of Bad by Western blot at 0.5 to 1 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Storage
BAD antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1213 - T24 Cell Lysate
- Cat. No. 1471 - Rat Thymus Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
17371773
Protein Accession Number
Q92934
This product belongs to the following categories:
Related Products
References- Cory S, Huang DCS, and Adams JM. The Bcl-2 family: roles in cell survival and oncogenesis. Oncogene 2003; 22:8590-607.
- Heiser D, Labi V, Erlacher M, et al. The Bcl-2 protein family and its role in the development of neoplastic disease. Exp. Geron. 2004; 39:1125-35.
- Ottilie S, Diaz JL, Horne W, et al. Dimerization properties of human BAD. Identification of a BH-3 domain and analysis of its binding to mutant BCL-2 and BCL-XL proteins. J. Biol. Chem. 1997; 272:30866-72.
- Zhou XM, Liu Y, Payne G, et al. Growth factors inactivate the cell death promoter BAD by phosphorylation of its BH3 domain on Ser155. J. Biol. Chem. 2000; 275:25046-51.
Datasheet 08-02W
|
|
