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Primary Antibodies
BACE2 Antibody
Background
Accumulation of the amyloid-β (αβ) plaque in the cerebral cortex is a critical event in the pathogenesis of Alzheimer’s disease. αβ peptide is generated by proteolytic cleavage of the β-amyloid protein precursor (APP) at β- and γ-sites by proteases. The long-sought β-secretase was recently identified by several groups independently and designated beta-site APP cleaving enzyme (BACE) and aspartyl protease 2 (Asp2) (1-4). BACE/Asp2 is a novel transmembrane aspartic protease and co-localizes with APP. A BACE homolog was recently cloned and designated BACE2, Asp1, DRAP (for Down region aspartic protease), and memapsin 1 (4-9). BACE2 also cleaves APP at b-site and at a different site within αβ (8). BACE2 locates on chromosome 21q22.3, the so-called ‘Down critical region’, suggesting that BACE2 and αβ may also contribute to the pathogenesis of Down syndrome (6,7)
Additional Names
BACE2 (CT), Asp2
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Description
Left: Western blot analysis of BACE2 in human heart tissue lysate in the absence (A) or presence (B) of blocking peptide with BACE2 antibody at 1 μg/ml.
Below: Immunohistochemistry of BACE2 in rat heart tissue with BACE2 antibody at 2 μg/ml.
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Other Product Images
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Source
BACE2 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human BACE2.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
BACE2 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human BACE2 (C-Terminus) Peptide (Cat. No. 2249P)
Application
BACE2 antibody can be used for detection of BACE2 by Western blot at 0.5 to 1 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 2249P - BACE2 Peptide
Storage
BACE2 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1301 - Human Heart Tissue Lysate
- Cat. No. 1461 - Rat Heart Tissue Lysate
Species Reactivity
H
Protein GI Number
6561812
Protein Accession Number
AAF17078
Short Description
(CT) Beta-site APP Cleaving Enzyme 2
This product belongs to the following categories:
Related Products
References- Vassar R, et al. β-secretase cleavage of Alzheimer's amyloid precursor protein by the transmembrane aspartic protease BACE. Science 1999;286:735-41
- Hussain I, et al. Identification of a novel aspartic protease (Asp 2) as β-secretase. Mol Cell Neurosci 1999;14:419-27
- Sinha S, et al. Purification and cloning of amyloid precursor protein β-secretase from human brain. Nature 1999;402:537-40
- Yan R, et al. Membrane-anchored aspartyl protease with Alzheimer's disease β-secretase activity. Nature 1999;402:533-7
Datasheet 08-02W
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