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Detection Sets
Avian Flu (H5N1) Protein Detection Set
Background
Influenza A virus is a major public health threat, killing more than 30,000 people per year in the USA. Novel influenza virus strains emerge periodically to which humans have little or no immunity, resulting in devastating pandemics. Influenza A can exist in a variety of animals; however it is in birds that all subtypes can be found. These subtypes are classified based on the combination of the virus coat glycoproteins hemagglutinin (HA) and neuraminidase (NA) subtypes. During 1997, an H5N1 avian influenza virus was determined to be the cause of death in 6 of 18 infected patients in Hong Kong. There was some evidence of human to human spread of this virus, but it is thought that the transmission efficiency was fairly low. Although it has been known that cleavage site and glycosylation patterns of the HA protein play important roles in determining the pathogenicity of H5 avian influenza viruses, it has only recently been shown that an additional glycosylation site within the globular head of the neuraminidase protein also contributes to the high virulence of the H5N1 virus. H5N1 hemagglutinin interacts with cell surface proteins containing oligosaccharides with terminal sialyl residues. Virus isolated from a human infected with the H5N1 strain in 1997 could bind to oligosaccharides from human as well as avian sources, indicating its species-jumping ability.
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Description
For images please see PDF data sheet
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Kit Contents
H5N1 Hemagglutinin Antibody (NT), Catalog No. 3427 (50μg)
H5N1 Hemagglutinin Antibody (IN), Catalog No. 3425 (50μg)
H5N1 Neuraminidase Antibody (CT), Catalog No. 3423 (50μg)
H5N1 Neuraminidase Antibody (IN), Catalog No. 3421 (50μg)
H5N1 Hemagglutinin Peptide (NT), Catalog No. 3427P (50μg)
H5N1 Hemagglutinin Peptide (IN), Catalog No. 3425P (50μg)
H5N1 Neuraminidase Peptide (CT), Cat. No. 3423P (50μg)
H5N1 Neuraminidase Peptide (IN), Catalog No. 3421P (50μg)
Source
Rabbit polyclonal antibodies were raised against peptides corresponding to amino acid sequences from each of the corresponding proteins. Efforts were made to use regions of each protein that appear to be relatively conserved across H5N1 sub-strains, but due to the inherent variability of the influenza virus, no guarantees can be made.
Application
These polyclonal antibodies can be used for detection of H5N1 Hemagglutinin or Neuraminidase proteins in bodily fluid or tissue by ELISA. Immunogenic peptides are provided as positive controls and to determine protein concentration. Each antibody will detect 10ng of its corresponding peptide. Immunoblot applications are pending. For research use only.
Tested Application
E
Storage
H5N1 antibodies are supplied as affinity chromatography purified IgG, in PBS containing 0.02% sodium azide. H5N1 antibodies should be stored at -4˚C. Peptides are supplied in PBS containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -4˚C, stable for one year.
Species Reactivity
see datasheet
Short Description
H5N1 Hemagglutinin (NT), H5N1 Hemagglutinin (IN), H5N1 Neuraminidase (CT), and H5N1 Neuramidinase (IN) along with the Complementary Peptides H5N1 Hemagglutinin (NT) peptide, H5N1 Hemagglutinin (IN) peptide, H5N1 Neuraminidase (CT) peptide, and H5N1 Neuramidinase (IN) peptide
This product belongs to the following categories:
Related Products
References- Thompson WW, Shay DK, Weintraub, et al. Mortality associated with influenza and respiratory syncytial virus in the United States. JAMA 2003; 289:179-186.
- Alexander DJ. A review of avian influenza. Proceedings of the European Society for Veterinary Virology (ESVV) Symposium on Influenza Viruses of Wild and Domestic Animals. Vet. Microbiol. 2000; 74:3-13.
- Shortridge KF, Zhou NN, Guan Y, et al. Characterization of avian H5N1 influenza viruses from poultry in Hong Kong. Virol. 1998; 252:331-342.
- Buxton Bridges C, Katz JM, Seto WH, et al. Risk of influenza A (H5N1) infection among health care workers exposed to patients with influenza A (H5N1), Hong Kong. J. Inf. Dis. 2000; 181:344-8.
Datasheet 0803-W
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